口腔胶原膜生产工艺的研究进展

doi:10.12337/zgkqzzxzz.2022.12.006

摘要/Abstract

摘要： 引导骨再生是口腔种植学中的重要技术,口腔胶原膜在其中发挥着关键作用。按照生产工艺可将口腔胶原膜分为脱细胞基质膜、纯化胶原膜、重组胶原膜等。目前市面上主要使用的是脱细胞基质膜。不同类型的胶原膜在生产流程中有相同与不同之处,不同的生产工艺也对胶原膜的性能产生了不同的影响,本文综述胶原膜的分类、生产流程、不同胶原膜的对比以及胶原膜的改良。

关键词: 引导组织再生, 引导骨再生, 胶原膜, 脱细胞基质, 生产工艺

Abstract: Guided bone regeneration (GBR) is an important technique in implant dentistry, in which oral collagen membranes play a key role. According to the production process, oral collagen membranes can be divided into acellular matrix membranes, purified collagen membranes and recombinant collagen membranes. At present, acellular membranes are main ones used clinically. Different types of collagen membranes have similarities and differences in production processes, and different production processes also have different effects on the properties of collagen membranes. This paper reviews the classification, production processes, comparison of different collagen membranes and the improvement of collagen membranes.

Key words: Guided tissue regeneration (GTR), Guided bone regeneration (GBR), Collagen membrane, Acellular matrix, Production process

舒倩怡,等宿玉成. 口腔胶原膜生产工艺的研究进展[J]. 中国口腔种植学杂志, 2022, 27(6): 358-364. DOI: 10.12337/zgkqzzxzz.2022.12.006

Shu Qianyi, Su Yucheng. Research advances in the production process of dental collagen membrane[J].Chinese Journal of Oral Implantology, 2022, 27(6): 358-364.DOI: 10.12337/zgkqzzxzz.2022.12.006.

参考文献

[1] Murray G, Holden R, Roschlau W.Experimental and clinical study of new growth of bone in a cavity[J]. Am J Surg, 1957, 93(3):385-387. DOI: 10.1016/0002-9610(57)90827-9.
[2] Nyman S, Lindhe J, Karring T, et al.New attachment following surgical treatment of human periodontal disease[J]. J Clin Periodontol, 1982, 9(4):290-296. DOI: 10.1111/j.1600-051x.1982.tb02095.x.
[3] Nyman S.Bone regeneration using the principle of guided tissue regeneration[J]. J Clin Periodontol, 1991, 18(6):494-498. DOI: 10.1111/j.1600-051x.1991.tb02322.x.
[4] Becker W, Becker BE, Berg L, et al.New attachment after treatment with root isolation procedures: report for treated class III and class II furcations and vertical osseous defects[J]. Int J Periodontics Restorative Dent, 1988, 8(3):8-23.
[5] 宿玉成. 口腔种植学词典 [M]. 北京:人民卫生出版社. 2020.
[6] Elgali I, Omar O, Dahlin C, et al.Guided bone regeneration: materials and biological mechanisms revisited[J]. Eur J Oral Sci, 2017, 125(5):315-337. DOI: 10.1111/eos.12364.
[7] Bornstein MM, Halbritter S, Harnisch H, et al.A retrospective analysis of patients referred for implant placement to a specialty clinic: indications, surgical procedures, and early failures[J]. Int J Oral Maxillofac Implants, 2008, 23(6):1109-1116.
[8] Sbricoli L, Guazzo R, Annunziata M, et al.Selection of collagen membranes for bone regeneration: a literature review[J]. Materials (Basel), 2020, 13(3):786. DOI: 10.3390/ma13030786.
[9] Sheikh Z, Qureshi J, Alshahrani AM, et al.Collagen based barrier membranes for periodontal guided bone regeneration applications[J]. Odontology, 2017, 105(1):1-12. DOI: 10.1007/s10266-016-0267-0.
[10] Caballé-Serrano J, Munar-Frau A, Ortiz-Puigpelat O, et al.On the search of the ideal barrier membrane for guided bone regeneration[J]. J Clin Exp Dent, 2018, 10(5):e477-e483. DOI: 10.4317/jced.54767.
[11] Calciolari E, Ravanetti F, Strange A, et al.Degradation pattern of a porcine collagen membrane in an in vivo model of guided bone regeneration[J]. J Periodontal Res, 2018, 53(3):430-439. DOI: 10.1111/jre.12530.
[12] Ventura RD, Padalhin AR, Park CM, et al.Enhanced decellularization technique of porcine dermal ECM for tissue engineering applications[J]. Mater Sci Eng C Mater Biol Appl, 2019, 104:109841. DOI: 10.1016/j.msec.2019.109841.
[13] Wu H, Yin G, Pu X, et al.Preliminary study on the antigen-removal from extracellular matrix via different decellularization[J]. Tissue Eng Part C Methods, 2022, 28(6):250-263. DOI: 10.1089/ten.TEC.2022.0025.
[14] Dearth CL, Keane TJ, Carruthers CA, et al.The effect of terminal sterilization on the material properties and in vivo remodeling of a porcine dermal biologic scaffold[J]. Acta Biomater, 2016, 33:78-87. DOI: 10.1016/j.actbio.2016.01.038.
[15] Reing JE, Brown BN, Daly KA, et al.The effects of processing methods upon mechanical and biologic properties of porcine dermal extracellular matrix scaffolds[J]. Biomaterials, 2010, 31(33):8626-8633. DOI: 10.1016/j.biomaterials.2010.07.083.
[16] Gilbert TW.Strategies for tissue and organ decellularization[J]. J Cell Biochem, 2012, 113(7):2217-2222. DOI: 10.1002/jcb.24130.
[17] Fu RH, Wang YC, Liu SP, et al.Decellularization and recellularization technologies in tissue engineering[J]. Cell Transplant, 2014, 23(4-5):621-630. DOI: 10.3727/096368914X678382.
[18] Crapo PM, Gilbert TW, Badylak SF.An overview of tissue and whole organ decellularization processes[J]. Biomaterials, 2011, 32(12):3233-3243. DOI: 10.1016/j.biomaterials.2011.01.057.
[19] Gilbert TW, Sellaro TL, Badylak SF.Decellularization of tissues and organs[J]. Biomaterials, 2006, 27(19):3675-3683. DOI: 10.1016/j.biomaterials.2006.02.014.
[20] YY/T 1794-2021,口腔胶原膜通用技术要求[S]. 2021.
[21] 张自强. 去端肽胶原蛋白生物材料结构设计、制备及应用[D].中国地质大学(北京), 2018.
[22] 闫建伟. 牙种植引导骨再生心包胶原膜的制备及理化性能研究[D].山东大学, 2017.
[23] 周建业, 胡盛寿, 任兵, 等. 牛心包脱细胞基质的研制[J]. 中国医学科学院学报, 2001, 23(2):193-195.
[24] 李玲慰, 胡晓文, 李斯日古楞, 等. 异种脱细胞真皮基质修复骨引导再生术后软组织缺损的临床观察[J]. 中国口腔种植学杂志, 2010, 15(2):80-84,95. DOI: 10.3969/j.issn. 1007-3957.2010.02.008.
[25] 胡秀莲, 林野, 邱立新, 等. 应用异体脱细胞真皮基质重建种植体周围附着牙龈的临床研究[J]. 实用口腔医学杂志, 2008, 24(4):540-543. DOI: 10.3969/j.issn. 1001-3733.2008.04.019.
[26] Jimbo R, Marin C, Witek L, et al.Bone morphometric evaluation around immediately placed implants covered with porcine-derived pericardium membrane: an experimental study in dogs[J]. Int J Biomater, 2012, 2012:279167. DOI: 10.1155/2012/279167.
[27] Rothamel D, Schwarz F, Fienitz T, et al.Biocompatibility and biodegradation of a native porcine pericardium membrane: results of in vitro and in vivo examinations[J]. Int J Oral Maxillofac Implants, 2012, 27(1):146-154.
[28] Liu X, Wu H, Lu F, et al.Fabrication of porous bovine pericardium scaffolds incorporated with bFGF for tissue engineering applications[J]. Xenotransplantation, 2020, 27(1):e12568. DOI: 10.1111/xen.12568.
[29] Mendoza-Novelo B, Avila EE, Cauich-Rodríguez JV, et al.Decellularization of pericardial tissue and its impact on tensile viscoelasticity and glycosaminoglycan content[J]. Acta Biomater, 2011, 7(3):1241-1248. DOI: 10.1016/j.actbio.2010.11.017.
[30] Annibali S, Bignozzi I, Sammartino G, et al.Horizontal and vertical ridge augmentation in localized alveolar deficient sites: a retrospective case series[J]. Implant Dent, 2012, 21(3):175-185. DOI: 10.1097/ID.0b013e31824ee3e9.
[31] Geistlich P, Eckmayer Z, Boyne P.Resorbable collagen membrane for use in guided tissue regeneration[P]. US:US5837278 A.1998.
[32] Meyer M.Processing of collagen based biomaterials and the resulting materials properties[J]. Biomed Eng Online, 2019, 18(1):24. DOI: 10.1186/s12938-019-0647-0.
[33] Bondioli E, Fini M, Veronesi F, et al.Development and evaluation of a decellularized membrane from human dermis[J]. J Tissue Eng Regen Med, 2014, 8(4):325-336. DOI: 10.1002/term.1530.
[34] Panahipour L, Kargarpour Z, Luza B, et al.TGF-β activity related to the use of collagen membranes: in vitro bioassays[J]. Int J Mol Sci, 2020, 21(18):6636. DOI: 10.3390/ijms21186636.
[35] Spinell T, Saliter J, Hackl B, et al.In-vitro cytocompatibility and growth factor content of GBR/GTR membranes[J]. Dent Mater, 2019, 35(7):963-969. DOI: 10.1016/j.dental.2019.03.011.
[36] Li Q, Uygun BE, Geerts S, et al.Proteomic analysis of naturally-sourced biological scaffolds[J]. Biomaterials, 2016, 75:37-46. DOI: 10.1016/j.biomaterials.2015.10.011.
[37] Hill RC, Calle EA, Dzieciatkowska M, et al.Quantification of extracellular matrix proteins from a rat lung scaffold to provide a molecular readout for tissue engineering[J]. Mol Cell Proteomics, 2015, 14(4):961-973. DOI: 10.1074/mcp.M114.045260.
[38] Lee JS, Mitulović G, Panahipour L, et al.Proteomic analysis of porcine-derived collagen membrane and matrix[J]. Materials (Basel), 2020, 13(22):5187. DOI: 10.3390/ma13225187.
[39] Pitaru S, Tal H, Soldinger M, et al.Partial regeneration of periodontal tissues using collagen barriers. Initial observations in the canine[J]. J Periodontol, 1988, 59(6):380-386. DOI: 10.1902/jop.1988.59.6.380.
[40] 周恬. 仿生鱼胶原膜制备和诱导组织再生及机制研究[D].上海交通大学, 2015.
[41] 王瑶. 纤维内外协同矿化胶原支架和胶原膜材料的构建及研究[D].天津医科大学, 2017.
[42] 杨珺, 肖仕初, 夏照帆. 海绵状胶原膜制备真皮支架及其生物活性研究[J]. 第二军医大学学报, 2002, 23(4):437-439. DOI: 10.3321/j.issn:0258-879X.2002.04.028.
[43] 孟宪敏, 赵淑惠, 陈翠真. 猪巩膜胶原的纯化及胶原膜制备的研究[J]. 中国药学杂志, 1998, (6):345. DOI: 10.3321/j.issn:1001-2494.1998.06.008.
[44] 李成章, 樊明文. 冻干交联胶原膜制备和特性分析 Ⅱ.小鼠皮下和人牙周袋内植入观察[J]. 口腔医学纵横, 1997, 2:67-69.
[45] 陈亮, 孟姝, 程国平, 等. 鱼鳞胶原膜对大鼠骨髓间充质干细胞黏附增殖及成骨分化的影响[J]. 中国组织工程研究, 2021, 25(16):2494-2499.
[46] Geistlich P.Resorbable extracellular matrix for reconstruction of bone[P]. Canada: CA 2419620C, 2015.
[47] 何越, 侯增淼, 李晓颖, 等. 重组胶原蛋白海绵的制备及性状表征[J]. 中国组织工程研究, 2019, 23(6):912-916. DOI: 10.3969/j.issn.2095-4344.1580.
[48] 刘斌, 雷云霆, 张静, 等. 重组胶原蛋白和明胶的研究进展[J]. 中国医药工业杂志, 2011, 42(11):863-867. DOI: 10.3969/j.issn.1001-8255.2011.11.016.
[49] Fertala A.Three decades of research on recombinant collagens: reinventing the wheel or developing new biomedical Products?[J]. Bioengineering (Basel), 2020, 7(4):155. DOI: 10.3390/bioengineering7040155.
[50] Yang C, Guo J, Wang J, et al.[Effects of two common acellular methods on the physicochemical properties of dermal acellular matrix][J]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi, 2021, 38(5):911-918. DOI: 10.7507/1001-5515.202103019.
[51] Rajab TK, O'Malley TJ, Tchantchaleishvili V. Decellularized scaffolds for tissue engineering: current status and future perspective[J]. Artif Organs, 2020, 44(10):1031-1043. DOI: 10.1111/aor.13701.
[52] Wu LC, Kuo YJ, Sun FW, et al.Optimized decellularization protocol including α-Gal epitope reduction for fabrication of an acellular porcine annulus fibrosus scaffold[J]. Cell Tissue Bank, 2017, 18(3):383-396. DOI: 10.1007/s10561-017-9619-4.
[53] Chu C, Deng J, Sun X, et al.Collagen membrane and immune response in guided bone regeneration: recent progress and perspectives[J]. Tissue Eng Part B Rev, 2017, 23(5):421-435. DOI: 10.1089/ten.TEB.2016.0463.
[54] Bunyaratavej P, Wang HL.Collagen membranes: a review[J]. J Periodontol, 2001, 72(2):215-229. DOI: 10.1902/jop.2001.72.2.215.
[55] Moses O, Vitrial D, Aboodi G, et al.Biodegradation of three different collagen membranes in the rat calvarium: a comparative study[J]. J Periodontol, 2008, 79(5):905-911. DOI: 10.1902/jop.2008.070361.
[56] 史佳巍, 李学敏, 刘玲蓉, 等. D-核糖交联胶原膜的性质研究[J]. 功能材料, 2009, 40(6):1013-1016. DOI: 10.3321/j.issn:1001-9731.2009.06.039.
[57] Speer DP, Chvapil M, Eskelson CD, et al.Biological effects of residual glutaraldehyde in glutaraldehyde-tanned collagen biomaterials[J]. J Biomed Mater Res, 1980, 14(6):753-764. DOI: 10.1002/jbm.820140607.
[58] Ferreira AM, Gentile P, Chiono V, et al.Collagen for bone tissue regeneration[J]. Acta Biomater, 2012, 8(9):3191-3200. DOI: 10.1016/j.actbio.2012.06.014.
[59] Tanaka S, Avigad G, Eikenberry EF, et al.Isolation and partial characterization of collagen chains dimerized by sugar-derived cross-links[J]. J Biol Chem, 1988, 263(33):17650-17657.
[60] Zubery Y, Goldlust A, Alves A, et al.Ossification of a novel cross-linked porcine collagen barrier in guided bone regeneration in dogs[J]. J Periodontol, 2007, 78(1):112-121. DOI: 10.1902/jop.2007.060055.
[61] Zubery Y, Nir E, Goldlust A.Ossification of a collagen membrane cross-linked by sugar: a human case series[J]. J Periodontol, 2008, 79(6):1101-1107. DOI: 10.1902/jop.2008.070421.
[62] Minabe M, Kodama T, Kogou T, et al.Different cross-linked types of collagen implanted in rat palatal gingiva[J]. J Periodontol, 1989, 60(1):35-43. DOI: 10.1902/jop.1989.60.1.35.
[63] Boekema BK, Vlig M, Olde Damink L, et al.Effect of pore size and cross-linking of a novel collagen-elastin dermal substitute on wound healing[J]. J Mater Sci Mater Med, 2014, 25(2):423-433. DOI: 10.1007/s10856-013-5075-2.
[64] Becker J, Al-Nawas B, Klein MO, et al.Use of a new cross-linked collagen membrane for the treatment of dehiscence-type defects at titanium implants: a prospective, randomized-controlled double-blinded clinical multicenter study[J]. Clin Oral Implants Res, 2009, 20(7):742-749. DOI: 10.1111/j.1600-0501.2008.01689.x.
[65] Wessing B, Lettner S, Zechner W.Guided Bone Regeneration with collagen membranes and particulate graft materials: a systematic review and meta-analysis[J]. Int J Oral Maxillofac Implants, 2018, 33(1):87-100. DOI: 10.11607/jomi.5461.
[66] Jiménez Garcia J, Berghezan S, Caramês J, et al.Effect of cross-linked vs non-cross-linked collagen membranes on bone: a systematic review[J]. J Periodontal Res, 2017, 52(6):955-964. DOI: 10.1111/jre.12470.
[67] Garcia J, Dodge A, Luepke P, et al.Effect of membrane exposure on guided bone regeneration: a systematic review and meta-analysis[J]. Clin Oral Implants Res, 2018, 29(3):328-338. DOI: 10.1111/clr.13121.
[68] Park SH, Lee KW, Oh TJ, et al.Effect of absorbable membranes on sandwich bone augmentation[J]. Clin Oral Implants Res, 2008, 19(1):32-41. DOI: 10.1111/j.1600-0501.2007.01408.x.
[69] Machtei EE.The effect of membrane exposure on the outcome of regenerative procedures in humans: a meta-analysis[J]. J Periodontol, 2001, 72(4):512-516. DOI: 10.1902/jop.2001.72.4.512.
[70] Gouk SS, Lim TM, Teoh SH, et al.Alterations of human acellular tissue matrix by gamma irradiation: histology, biomechanical property, stability, in vitro cell repopulation, and remodeling[J]. J Biomed Mater Res B Appl Biomater, 2008, 84(1):205-217. DOI: 10.1002/jbm.b.30862.
[71] 汤克勇. 胶原物理与化学 [M]. 北京: 科学出版社, 2012.
[72] Alberts B, Johnson A, Lewis J, et al.Molecular biology of the cell[M]. 6th ed. New York: Garland Science, 2015.
[73] Stone KevinR,Shu-tung Li.Prosthetic artilage[P]. US:WO1993011723A1, 1993.
[74] Báez J, Olsen D, Polarek JW.Recombinant microbial systems for the production of human collagen and gelatin[J]. Appl Microbiol Biotechnol, 2005, 69(3):245-252. DOI: 10.1007/s00253-005-0180-x.
[75] 侯增淼, 李晓颖, 李敏, 等. 重组人源性胶原蛋白的制备及表征[J]. 生物工程学报, 2019, 35(2):319-326. DOI: 10.13345/j.cjb.180266.
[76] Brightman AO, Rajwa BP, Sturgis JE, et al. Time-lapse confocal reflection microscopy of collagen fibrillogenesis and extracellular matrix assembly in vitro[J]. Biopolymers, 2000, 54(3):222-234. DOI: 10.1002/1097-0282(200009)54:3<222:AID-BIP80>3.0.CO;2-K.
[77] Liu S, Zhang H, Zhang X, et al.Synergistic angiogenesis promoting effects of extracellular matrix scaffolds and adipose-derived stem cells during wound repair[J]. Tissue Eng Part A, 2011, 17(5-6):725-739. DOI: 10.1089/ten.TEA.2010.0331.
[78] Liu TJ, Zhou JN, Guo LH.Impact of different regenerative techniques and materials on the healing outcome of endodontic surgery: a systematic review and meta-analysis[J]. Int Endod J, 2021, 54(4):536-555. DOI: 10.1111/iej.13440.
[79] Martin-Thomé H, Bourdin D, Strube N, et al.Clinical safety of a new synthetic resorbable dental membrane: a case series study[J]. J Oral Implantol, 2018, 44(2):138-145. DOI: 10.1563/aaid-joi-D-17-00042.
[80] Oh TJ, Meraw SJ, Lee EJ, et al.Comparative analysis of collagen membranes for the treatment of implant dehiscence defects[J]. Clin Oral Implants Res, 2003, 14(1):80-90. DOI: 10.1034/j.1600-0501.2003.140111.x.
[81] Cheng CF, Lee YY, Chi LY, et al.Bacterial penetration through antibiotic-loaded guided tissue regeneration membranes[J]. J Periodontol, 2009, 80(9):1471-1478. DOI: 10.1902/jop.2009.090044.
[82] Gilberto Goiddisa, Sousab Mhd.Characterization and in vitro release studies of tetra cycline and rolite tracycline imobilized on anionic collagen membranes[J]. Materials Research, 2009, 12(1): 69-74.
[83] 曹钰彬, 刘畅, 潘韦霖, 等. 引导骨再生屏障膜改良的研究进展[J]. 华西口腔医学杂志, 2019, 37(3):325-329. DOI: 10.7518/hxkq.2019.03.019.
[84] Sela MN, Babitski E, Steinberg D, et al.Degradation of collagen-guided tissue regeneration membranes by proteolytic enzymes of porphyromonas gingivalis and its inhibition by antibacterial agents[J]. Clin Oral Implants Res, 2009, 20(5):496-502. DOI: 10.1111/j.1600-0501.2008.01678.x.
[85] Mizuno D, Kagami H, Mizuno H, et al.Bone regeneration of dental implant dehiscence defects using a cultured periosteum membrane[J]. Clin Oral Implants Res, 2008, 19(3):289-294. DOI: 10.1111/j.1600-0501.2007.01452.x.
[86] 赖春花, 周磊, 宁晖丽, 等. 重组人骨形成蛋白-2结合胶原膜在兔颅骨引导骨再生模型中的成骨效应研究[J]. 中国实用口腔科杂志, 2016, 9(4):228-232,236. DOI: 10.7504/kq.2016.04.009.
[87] Turri A, Elgali I, Vazirisani F, et al.Guided bone regeneration is promoted by the molecular events in the membrane compartment[J]. Biomaterials, 2016, 84:167-183. DOI: 10.1016/j.biomaterials.2016.01.034.
[88] Londono R, Badylak SF.Biologic scaffolds for regenerative medicine: mechanisms of in vivo remodeling[J]. Ann Biomed Eng, 2015, 43(3):577-592. DOI: 10.1007/s10439-014-1103-8.