壳聚糖基温敏凝胶膜体内引导骨再生性能研究

doi:10.12337/zgkqzzxzz.2019.03.001

中国口腔种植学杂志 ›› 2019, Vol. 24 ›› Issue (1): 1-4.DOI: 10.12337/zgkqzzxzz.2019.03.001

• 专题研究 • 下一篇

壳聚糖基温敏凝胶膜体内引导骨再生性能研究

梁杰, 周倜, 崔军, 许胜, 徐欣

264001 烟台烟台市口腔医院种植科(梁杰, 周倜);济南济南市口腔医院种植中心(崔军);济南山东大学口腔医学院种植中心(徐欣);济南山东省口腔生物医学重点实验室(徐欣)

出版日期:2019-03-10 发布日期:2021-09-07
通讯作者: 徐欣
基金资助:
烟台市科技发展计划项目(2013ws248); 山东省优秀中青年科学家科研奖励基金计划项目(BS2013YY056)

The study on properties of guided bone regeneration of new type chitosan-based thermosensitive hydrogel membranes in vivo

LIANG Jie, ZHOU Ti, CUI Jun, et al

Department of Implantology, Yantai Stomatological Hospital,Shandong University, Yantai 264001, Shandong Province, China

Online:2019-03-10 Published:2021-09-07

摘要/Abstract

摘要： 目的： 观察壳聚糖/β-甘油磷酸钠(CS/β-GP,chitosan/β-glycerophosphate salt)温敏凝胶复合膜及其负载釉基质蛋白(EMPs,enamel matrix proteins)体内引导骨再生的生物特性。方法： 体外合成CS/β-GP温敏凝胶复合膜并负载EMPs。10只健康成年雄性Wistar大鼠随机分为A、B两组,分别为实验1个月和2个月组。大鼠颅骨中线两侧制备直径5mm的极量骨缺损(critical size defect, CSD);左侧为实验侧,覆盖CS/β-GP(1.0g)复合膜负载EMPs,右侧覆盖单纯CS/β-GP复合膜为对照侧。通过大体观察、X线新生骨密度测量、HE染色等方法比较两组复合膜修复骨缺损的情况。结果： 测量1个月、2个月双侧骨密度百分比,差异具有统计学意义(P< 0.05);HE染色发现,术后1个月双侧均有少量新骨生成;术后2个月,实验侧内的新骨生成增加且更致密、趋于成熟。结论： 该种类CS/β-GP复合膜具有引导骨再生的特性,负载生物活性因子后能明显加速骨愈合。

关键词: 壳聚糖, β-甘油磷酸钠, 釉基质蛋白, 温敏凝胶, 引导骨再生

Abstract: Objective： To observe chitosan/β-glycerophosphate salt(CS/β-GP) thermosensitive hydrogel mem-branes loaded enamel matrix proteins and detect the guided bone regeneration properties. Methods： CS/β-GP membranes were synthesized in vitro and loaded with EMPs. 10 male and healthy Wistar rats wererandomly divided into two groups(A and B). Critical size defect(CSD) in a diameter of 5mm was created onboth sides of the skull of the rats. The experimental side on the left was covered by CS/β-GP(1.0g) com-posite membrane loaded EMPs on the left and the control side was covered by CS/β-GP (1.0g) membraneon the right. The bone reparation of the groups on both sides was compared by observation, density mea-surement of newly formed bone and hematoxylin-eosin staining. Results： The percentage of bilateralbone density measured at 1 month and 2 months had statistical significance(P< 0.05). HE staining showedthat there were a few new bone formation on both sides at 1 month after operation. At 2 months after opera-tion, the new bone formation in the experimental side increased and became more compact and mature.Conclusion： This kind of CS/β-GP composite membrane has the characteristics of guided bone regener-ation, and can significantly accelerate bone healing after loading bioactive factors.

Key words: chitosan, β-glycerophosphate salt, enamel matrix proteins, thermosensitive hydrogel, guided bone regeneration

中图分类号:

R783.6

梁杰,周倜,崔军,等. 壳聚糖基温敏凝胶膜体内引导骨再生性能研究[J]. 中国口腔种植学杂志, 2019, 24(1): 1-4. DOI: 10.12337/zgkqzzxzz.2019.03.001

LIANG Jie, ZHOU Ti, CUI Jun, et al. The study on properties of guided bone regeneration of new type chitosan-based thermosensitive hydrogel membranes in vivo[J].Chinese Journal of Oral Implantology, 2019, 24(1): 1-4.DOI: 10.12337/zgkqzzxzz.2019.03.001.

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壳聚糖基温敏凝胶膜体内引导骨再生性能研究

The study on properties of guided bone regeneration of new type chitosan-based thermosensitive hydrogel membranes in vivo

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