2型糖尿病个体进行即刻种植即刻修复预后的动物实验研究

doi:10.12337/zgkqzzxzz.2024.08.004

中国口腔种植学杂志 ›› 2024, Vol. 29 ›› Issue (4): 311-319.DOI: 10.12337/zgkqzzxzz.2024.08.004

• “全身系统性疾病与牙种植”重点栏目 • 上一篇下一篇

2型糖尿病个体进行即刻种植即刻修复预后的动物实验研究

赵国强^1,2, 宋应亮¹

¹口颌系统重建与再生全国重点实验室国家口腔疾病临床医学研究中心陕西省口腔生物工程工程技术研究中心空军军医大学第三附属医院口腔种植科,西安 710032;
²空军杭州特勤疗养中心疗养五区疗养一科,无锡 214128,江苏

收稿日期:2024-04-28 出版日期:2024-08-30 发布日期:2024-08-16
通讯作者: 宋应亮,Email：songyingliang@163.com,电话：029-84776454
作者简介:赵国强口腔种植学博士、主治医师,研究方向：糖尿病患者口腔种植,即刻种植修复; 宋应亮口腔种植学博士、教授、主任医师,研究方向：糖尿病患者口腔种植,复杂条件下口腔种植,激光治疗种植体周炎等

Animal experiment of prognosis of immediate implant placement and restoration in individuals with T2DM

Zhao Guoqiang^1,2, Song Yingliang¹

¹State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Oral Implants, The Third Hospital Affiliated of Air Force Military Medical University, Xi' an 710032, China;
²Air Force Special Service Recuperation Center 5th Sanatorium 1st Recuperation Department, Wuxi 214128, Jiangsu, China

Received:2024-04-28 Online:2024-08-30 Published:2024-08-16
Contact: Song Yingliang, Email: songyingliang@163.com, Tel: 0086-29-84776454

摘要/Abstract

摘要： 目的通过建立动物模型,验证2型糖尿病个体即刻种植即刻修复的可行性,并评估其预后。方法应用新西兰兔构建2型糖尿病动物模型作为实验组,以健康新西兰兔为对照组,2组均进行即刻种植即刻修复,通过Micro-CT扫描、硬组织切片染色及分析、实时定量PCR检测炎症相关因子,对比2组的预后及组织学改建有无差异。结果 2组实验兔即刻种植即刻修复后种植体留存率均为90%,术后早期（2个月内）实验组种植体周骨质在各项微观参数中与对照组存在显著差异,同时术后1个月实验组种植体周软组织内IL-6、IL-33与TNF-α的表达显著高于对照组。结论动物实验表明2型糖尿病动物模型应用即刻种植即刻修复后具有较好的安全性,但种植体早期骨结合形成缓慢、种植体周软组织炎症更为明显。

关键词: 口腔种植, 即刻种植, 即刻修复, 动物实验, 2型糖尿病

Abstract: Objective To investigate the feasibility and prognosis of immediate implant placement and immediate restoration in patients with type 2 diabetes by establishing an animal model. Methods The type 2 diabetic animal model was established using New Zealand rabbits as the experimental group and healthy New Zealand rabbits as the control group. Both groups underwent immediate implantation and immediate repair. Micro-CT scanning, hard tissue section, and detection of relative inflammatory factors by real-time quantitative PCR were used to compare prognosis and histological remodeling between the two groups. Results The success rate for immediate implant placement and immediate restoration was 90% in both groups. In the early postoperative period (1~2 months), the peri-implant bone of the experimental rabbits in the T2DM group was significantly different from the control group in various microscopic parameters, in the meantime, the expressions of IL-6, IL-33 and TNF-α were significantly higher than those of the control group. Conclusion Animal experiments have shown that the immediate implant placement and immediate prosthesis are safe in type 2 diabetic animal models. However, attention should be paid to the slow formation of osseointegration and obvious inflammation of the soft tissue around the implant in the early stage.

Key words: Dental implant, Immediate implant placement, Immediate restoration, Animal experiment, Type 2 diabetes mellitus

赵国强,等宋应亮. 2型糖尿病个体进行即刻种植即刻修复预后的动物实验研究[J]. 中国口腔种植学杂志, 2024, 29(4): 311-319. DOI: 10.12337/zgkqzzxzz.2024.08.004

Zhao Guoqiang, Song Yingliang. Animal experiment of prognosis of immediate implant placement and restoration in individuals with T2DM[J].Chinese Journal of Oral Implantology, 2024, 29(4): 311-319.DOI: 10.12337/zgkqzzxzz.2024.08.004.

参考文献

[1] Alasqah M, Mokeem S, Alrahlah A, et al.Periodontal parameters in prediabetes, type 2 diabetes mellitus, and non-diabetic patients[J]. Braz Oral Res, 2018,32:e81. DOI: 10.1590/1807-3107bor-2018.vol32.0081.
[2] Raju K, Taylor GW, Tahir P, et al.Association of tooth loss with morbidity and mortality by diabetes status in older adults: a systematic review[J]. BMC Endocr Disord, 2021,21(1):205. DOI: 10.1186/s12902-021-00830-6.
[3] Power DJ, Sambrook PJ, Goss AN.The healing of dental extraction sockets in insulin-dependent diabetic patients: a prospective controlled observational study[J]. Aust Dent J, 2019,64(1):111-116. DOI: 10.1111/adj.12669.
[4] Wang F, Song YL, Li DH, et al.Type 2 diabetes mellitus impairs bone healing of dental implants in GK rats[J]. Diabetes Res Clin Pract, 2010,88(1):e7-e9. DOI: 10.1016/j.diabres.2010.01.017.
[5] 陆添, 吴蔚, 刘敏. Ⅱ型糖尿病患者种植牙二期手术后牙龈愈合状况研究[J].健康研究,2017,37(4):417-419,423. DOI: 10.3969/j.issn.1674-6449.2017.04.017.
[6] Arora H, Ivanovski S.Clinical and aesthetic outcomes of immediately placed single-tooth implants with immediate vs. delayed restoration in the anterior maxilla: a retrospective cohort study[J]. Clin Oral Implants Res, 2018,29(3):346-352. DOI: 10.1111/clr.13125.
[7] Alqutaibi AY, Hussain BS, Alobodi AK.Immediate and conventional loading of a single dental implant placed in the esthetic zone may yield comparable clinical outcomes[J]. J Evid Based Dent Pract, 2020,20(4):101492. DOI: 10.1016/j.jebdp.2020.101492.
[8] Slagter KW, Raghoebar GM, Hentenaar D, et al.Immediate placement of single implants with or without immediate provisionalization in the maxillary aesthetic region: a 5-year comparative study[J]. J Clin Periodontol, 2021,48(2):272-283. DOI: 10.1111/jcpe.13398.
[9] Shi S, Song S, Liu X, et al.Construction and performance of exendin-4-loaded chitosan-PLGA microspheres for enhancing implant osseointegration in type 2 diabetic rats[J]. Drug Deliv, 2022,29(1):548-560. DOI: 10.1080/10717544.2022.2036873.
[10] Gadicherla S, Smriti K, Roy S, et al.Comparison of extraction socket healing in non-diabetic, prediabetic, and type 2 diabetic patients[J]. Clin Cosmet Investig Dent, 2020,12:291-296. DOI: 10.2147/CCIDE.S264196.
[11] Mitsias M, Siormpas K, Pistilli V, et al.Immediate, early (6 weeks) and delayed loading (3 months) of single, partial and full fixed implant supported prostheses: 1-year post-loading data from a multicentre randomised controlled trial[J]. Eur J Oral Implantol, 2018,11(1):63-75.
[12] Al Hezaimi K, Naghshbandi J, Nooh N, et al.Buccal bone remodeling around immediate implants in STZ-induced diabetic dogs: a histologic and microcomputed tomographic analysis[J]. Int J Periodontics Restorative Dent, 2021,41(5):683-690. DOI: 10.11607/prd.4589.
[13] Andrade C, Paz J, de Melo GS, et al. Survival rate and peri-implant evaluation of immediately loaded dental implants in individuals with type 2 diabetes mellitus: a systematic review and meta-analysis[J]. Clin Oral Investig, 2022,26(2):1797-1810. DOI: 10.1007/s00784-021-04154-6.
[14] Ibraheem EMA, Hammad HGH, El-Sisy AME.Comparing marginal bone height changes around immediately and delayed implant-retained mandibular overdentures in controlled diabetic patients: a randomized clinical study[J].Bull Natl Res Cent, 2019, 43(1):1-8. DOI: 10.1186/s42269-019-0228-1.
[15] 王宗保, 姚峰, 吴端生, 等. 高糖高脂致家兔糖尿病的实验模型探讨[J].中国实验动物学报,2003,11(2):103-106. DOI: 10.3969/j.issn.1005-4847.2003.02.010.
[16] Lagunov VL, Sun J, George R.Evaluation of biologic implant success parameters in type 2 diabetic glycemic control patients versus health patients: a meta-analysis[J]. J Investig Clin Dent, 2019,10(4):e12478. DOI: 10.1111/jicd.12478.
[17] Al-Shibani N, Al-Aali KA, Al-Hamdan RS, et al.Comparison of clinical peri-implant indices and crestal bone levels around narrow and regular diameter implants placed in diabetic and non-diabetic patients: a 3-year follow-up study[J]. Clin Implant Dent Relat Res, 2019,21(2):247-252. DOI: 10.1111/cid.12712.
[18] Power DJ, Sambrook PJ, Goss AN.The healing of dental extraction sockets in insulin-dependent diabetic patients: a prospective controlled observational study[J]. Aust Dent J, 2019,64(1):111-116. DOI: 10.1111/adj.12669.
[19] Wang F, Song YL, Li DH, et al.Type 2 diabetes mellitus impairs bone healing of dental implants in GK rats[J]. Diabetes Res Clin Pract, 2010,88(1):e7-e9. DOI: 10.1016/j.diabres.2010.01.017.
[20] Andrade C, Paz J, de Melo GS, et al. Survival rate and peri-implant evaluation of immediately loaded dental implants in individuals with type 2 diabetes mellitus: a systematic review and meta-analysis[J]. Clin Oral Investig, 2022,26(2):1797-1810. DOI: 10.1007/s00784-021-04154-6.
[21] Al Amri MD, Alfarraj Aldosari AM, Al-Johany SS, et al.Comparison of clinical and radiographic status around immediately loaded versus conventional loaded implants placed in patients with type 2 diabetes: 12- and 24-month follow-up results[J]. J Oral Rehabil, 2017,44(3):220-228. DOI: 10.1111/joor.12466.
[22] Al Amri MD, Kellesarian SV, Al-Kheraif AA, et al.Effect of oral hygiene maintenance on hba1c levels and peri-implant parameters around immediately-loaded dental implants placed in type-2 diabetic patients: 2 years follow-up[J]. Clin Oral Implants Res, 2016,27(11):1439-1443. DOI: 10.1111/clr.12758.
[23] Juncar RI, Precup AI, Juncar M.Immediate implant-prosthetic dental rehabilitation of patients with diabetes using four immediately loaded dental implants: a pilot study[J]. J Int Med Res, 2020,48(3):300060519897195. DOI: 10.1177/0300060519897195.
[24] 王力锋, 方开秀, 许晓茹, 等. 2型糖尿病大鼠颅骨极限骨缺损的实验研究[J].实用口腔医学杂志,2015,(2):157-161. DOI: 10.3969/j.issn.1001-3733.2015.02.02.
[25] Yang M, Xie J, Lei X, et al.Tubeimoside I suppresses diabetes-induced bone loss in rats, osteoclast formation, and rankl-induced nuclear factor-κB pathway[J]. Int Immunopharmacol, 2020,80:106202. DOI: 10.1016/j.intimp.2020.106202.
[26] Akram Z, Rahim ZH, Taiyeb-Ali TB, et al.Resistin as potential biomarker for chronic periodontitis: a systematic review and meta-analysis[J]. Arch Oral Biol, 2017,73:311-320. DOI: 10.1016/j.archoralbio.2016.08.016.
[27] Zhang Y, Jia R, Zhang Y, et al.Effect of non-surgical periodontal treatment on cytokines/adipocytokines levels among periodontitis patients with or without obesity: a systematic review and meta-analysis[J]. BMC Oral Health, 2023,23(1):717. DOI: 10.1186/s12903-023-03383-3.
[28] Sun R, Liang C, Sun Y, et al.Effects of metformin on the osteogenesis of alveolar BMSCs from diabetic patients and implant osseointegration in rats[J]. Oral Dis, 2022,28(4):1170-1180. DOI: 10.1111/odi.13808.
[29] Wang X, Jiang L, Shao X.Association analysis of insulin resistance and osteoporosis risk in chinese patients with T2DM[J]. Ther Clin Risk Manag, 2021,17:909-916. DOI: 10.2147/TCRM.S328510.
[30] King S, Klineberg I, Levinger I, et al.The effect of hyperglycaemia on osseointegration: a review of animal models of diabetes mellitus and titanium implant placement[J]. Arch Osteoporos, 2016,11(1):29. DOI: 10.1007/s11657-016-0284-1.
[31] Sam L, Chattipakorn S, Khongkhunthian P.Osseointegration of maxillary dental implants in diabetes mellitus patients: a randomized clinical trial human histomorphometric study[J].Appl Sci, 2020, 10(19):6762. DOI: 10.3390/app10196762.
[32] Yu T, Acharya A, Mattheos N, et al.Molecular mechanisms linking peri-implantitis and type 2 diabetes mellitus revealed by transcriptomic analysis[J]. PeerJ, 2019,7:e7124. DOI: 10.7717/peerj.7124.
[33] Guzeldemir E, Gunhan M, Ozcelik O, et al.Interleukin-1 and tumor necrosis factor-alpha gene polymorphisms in turkish patients with localized aggressive periodontitis[J]. J Oral Sci, 2008,50(2):151-159. DOI: 10.2334/josnusd.50.151.
[34] Mokeem S, Alfadda SA, Al-Shibani N, et al.Clinical and radiographic peri-implant variables around short dental implants in type 2 diabetic, prediabetic, and non-diabetic patients[J]. Clin Implant Dent Relat Res, 2019,21(1):60-65. DOI: 10.1111/cid.12691.

2型糖尿病个体进行即刻种植即刻修复预后的动物实验研究

Animal experiment of prognosis of immediate implant placement and restoration in individuals with T2DM

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	汤祎熳, 邱立新. 器官移植患者接受种植治疗时的临床思考[J]. 中国口腔种植学杂志, 2024, 29(4): 297-302.
[2]	张燕婷, 汪伟, 陈莉, 赵雯, 周炜. ASA Ⅲ级心血管疾病患者口腔种植修复的围手术期风险评估与管理[J]. 中国口腔种植学杂志, 2024, 29(4): 328-335.
[3]	邱韵, 王宇蓝, 张玉峰. 骨质疏松症对牙种植的影响及临床考量[J]. 中国口腔种植学杂志, 2024, 29(4): 336-341.
[4]	王一茗, 李昕茹, 滕微微, 马骏驰, 周立波. 口腔种植机器人在临床前及临床研究应用精度的Meta分析[J]. 中国口腔种植学杂志, 2024, 29(4): 362-370.
[5]	李歆, 刘开政, 武诗语, 刘远翔, 黄宝鑫, 乔威, 潘浩波, 陈卓凡. 口腔种植治疗中骨再生材料的研究现状[J]. 中国口腔种植学杂志, 2024, 29(4): 371-377.
[6]	陈江, 丘雨蓓. 数字化技术在口腔种植骨增量中的应用及研究进展[J]. 中国口腔种植学杂志, 2024, 29(3): 252-257.
[7]	赵宁, 李晓杰, 寇霓, 马国武. 数字化辅助美学区穿埋伏牙即刻种植一例[J]. 中国口腔种植学杂志, 2024, 29(3): 266-271.
[8]	王铭阳, 黄海涛. 质子泵抑制剂对口腔种植体骨结合影响的研究进展[J]. 中国口腔种植学杂志, 2024, 29(3): 277-283.
[9]	王琦, 杨波, 刘璐, 李翠英, 王心彧. 不同颌骨密度下种植体设计对其骨界面应力分布影响的有限元分析[J]. 中国口腔种植学杂志, 2024, 29(2): 159-168.
[10]	赵文博, 李昕茹, 苏航, 滕微微, 蔡雯昱, 周立波. 人工智能在下颌磨牙区即刻种植测量分析中的可行性研究[J]. 中国口腔种植学杂志, 2024, 29(2): 178-183.
[11]	宣岩, 郝俊燕, 安阳, 张婷, 张文静, 李丹. 基于“互联网+”健康管理模式在口腔种植患者围手术期的应用[J]. 中国口腔种植学杂志, 2024, 29(1): 56-61.
[12]	孟怡麟, 瞿晓辉, 滕新亚, 李如华, 毕立锋, 邹德荣. 全牙列即刻种植即刻负荷的机器人复杂种植[J]. 中国口腔种植学杂志, 2024, 29(1): 70-76.
[13]	刘鲁鹏, 赵歌, 杨云东, 肖慧娟. 左上中切牙种植修复后低咬合1例[J]. 中国口腔种植学杂志, 2024, 29(1): 77-81.
[14]	高乾程, 李新东, 曹明国, 刘云峰. 基于CBCT的深度学习辅助解剖结构分割在口腔种植中的应用[J]. 中国口腔种植学杂志, 2024, 29(1): 82-86.
[15]	徐淑兰, 朱元希. 种植体周炎的治疗决策和预后评估[J]. 中国口腔种植学杂志, 2023, 28(6): 394-401.