3D打印仿生钛网及聚氨酯涂层对钛网暴露后骨增量效果的生物学性能研究

doi:10.12337/zgkqzzxzz.2023.12.009

中国口腔种植学杂志 ›› 2023, Vol. 28 ›› Issue (6): 444-450.DOI: 10.12337/zgkqzzxzz.2023.12.009

3D打印仿生钛网及聚氨酯涂层对钛网暴露后骨增量效果的生物学性能研究

韩振佳¹, 王心彧¹, 董美希¹, 张亮¹, 韩泽奎¹, 宿玉成^1,2,3, 臧旖欣¹, 段峰¹

¹黑龙江省口腔生物材料与临床应用重点实验室佳木斯大学口腔医学工程实验中心佳木斯大学附属口腔医学院 154002;
²北京瑞城口腔医院北京口腔种植培训中心 100032;
³中国医学科学院北京协和医院口腔种植中心 100032

收稿日期:2023-06-16 出版日期:2023-12-30 发布日期:2024-01-04
通讯作者: 臧旖欣,Email：717173575@qq.com,电话：0454-8627689;段峰,Email：duanfeng60@sohu.com,电话：0454-8627689
作者简介:韩振佳,主治医师、硕士研究生,研究方向：数字化口腔种植、种植辅助设备研发; 臧旖欣,主治医师、硕士研究生,研究方向：种植辅助设备研发、牙周病学; 段峰, 副教授、主任医师、硕士研究生导师,研究方向：数字化口腔种植
基金资助:
黑龙江省自然科学基金（LH2022H089）; 佳木斯大学青年创新人才培养支持计划项目（JMSUQP2022028）

Biological properties of 3D printed bionic titanium mesh and polyurethane coating on the effect of bone augmentation after titanium mesh exposure

Han Zhenjia¹, Wang Xinyu¹, Dong Meixi¹, Zhang Liang¹, Han Zekui¹, Su Yucheng^1,2,3, Zang Yixin¹, Duan Feng¹

¹Key Laboratory of Oral Biomaterials and Clinical Applications of Heilongjiang Province, Stomatology Engineering Experimental Center of Jiamusi University, School of Stomatology, Jiamusi University, Jiamusi 154002, Heilongjiang, China;
²Beijing Citident Hospital of Stomatology, Beijing Implant Training College (BITC), Beijing 100032, China;
³Dental Implant Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100032,China

Received:2023-06-16 Online:2023-12-30 Published:2024-01-04
Contact: Zang Yixin, Email: 717173575@qq.com, Tel: 0086-454-8627689; Duan Feng, Email: duanfeng@sohu.com, Tel: 0086-454-8627689
Supported by:
Natural Science Foundation of Heilongjiang Province (LH2022H089); Jiamusi University Youth Innovative Talent Training Support Program (JMSUQP2022028)

摘要/Abstract

摘要： 目的观察蛛网型个性化钛网暴露后的骨增量效果,并探究对钛网进行聚氨酯涂层是否可降低感染率。方法实验选取6只比格犬,对犬下颌双侧第二、三前臼齿虚拟拔除,构建骨缺损模型,设计、打印截骨导板及蛛网结构个性化钛网,分别对钛网进行酸蚀及聚氨酯涂层处理。对比格犬双侧下颌骨行牙槽骨骨缺损引导骨再生（guided bone regeneration,GBR）,术后2周在术区滴食诱食剂建立钛网暴露模型,3个月处死6只比格犬,通过观察、CBCT分析、体视显微镜观察分析各组钛网暴露后牙槽骨骨增量效果。结果 12个钛网暴露位点的3种结果：①钛网脱落,软组织愈合;②钛网在位,暴露持续存在;③钛网在位,软组织愈合。其中2个酸蚀位点及1个聚氨酯涂层位点出现不同程度感染症状。CBCT、体积比计算及体视显微镜观察得出：钛网脱落组成骨35%±13%;钛网暴露愈合组48%±8%的成骨明显高于暴露持续存在组的27%±10%（P<0.05）。钛网暴露后愈合组高于钛网暴露后脱落组（P>0.05）。结论个性化钛网早期暴露后依旧可以形成新生骨,暴露后愈合的成骨效果最佳。经聚氨酯涂层后感染率稍低于酸蚀组,两组的差异为16.6%。

关键词: 引导骨再生, 个性化钛网, 仿生蛛网孔隙结构, 钛网暴露

Abstract: Objective To observe the bone augmentation effect of spiderweb-shaped customized titanium mesh after exposure, and to investigate whether polyurethane coating of titanium mesh could reduce the infection rate. Methods Six Beagle dogs were selected, and the second and third premolars on both sides of the mandibles were virtually removed to construct the bone defect models. The spiderweb-structured customized titanium meshes and osteotomy guides were designed and printed. The titanium meshes were treated with acid etching and polyurethane coating, respectively. Guided bone regeneration (GBR) was performed in the mandibles of Beagle dogs bilaterally. The titanium mesh exposure models were established by feeding bait 2 weeks after GBR. Six Beagle dogs were executed 3 months later. The effect of alveolar bone augmentation after titanium mesh exposure in each group was analyzed by gross observation, CBCT analysis and microscope observation. Results There were 3 kinds of results at 12 sites of titanium mesh exposure: ① Titanium mesh detachment with soft tissue healing;② Titanium mesh in place with persistent exposure; ③ Titanium mesh in place with soft tissue healing. Among them, 2 acid etching sites and 1 polyurethane coating site showed different degrees of infection symptoms. The results of CBCT volume ratio calculation and microscope showed that 35% ± 13% osteogenesis in the titanium mesh detachment group, and 48% ± 8% osteogenesis in the titanium mesh exposed healing group was significantly higher than the 27% ± 10% in the exposed persistence group (P<0.05). The titanium mesh exposed healing group was higher than the titanium mesh detachment group (P>0.05). Conclusion Customized titanium mesh can still form new bone after early exposure, the best osteogenesis results were achieved with post-exposure healing. The infection rate after polyurethane coating was slightly lower than that of acid etching group, and the difference between the two groups was 16.6%.

Key words: Guided bone regeneration, Customized titanium mesh, Bionic spiderweb pore structure, Titanium mesh exposure

韩振佳,王心彧,董美希,等. 3D打印仿生钛网及聚氨酯涂层对钛网暴露后骨增量效果的生物学性能研究[J]. 中国口腔种植学杂志, 2023, 28(6): 444-450. DOI: 10.12337/zgkqzzxzz.2023.12.009

Han Zhenjia, Wang Xinyu, Dong Meixi, Zhang Liang, Han Zekui, Su Yucheng, Zang Yixin, Duan Feng. Biological properties of 3D printed bionic titanium mesh and polyurethane coating on the effect of bone augmentation after titanium mesh exposure[J].Chinese Journal of Oral Implantology, 2023, 28(6): 444-450.DOI: 10.12337/zgkqzzxzz.2023.12.009.

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3D打印仿生钛网及聚氨酯涂层对钛网暴露后骨增量效果的生物学性能研究

Biological properties of 3D printed bionic titanium mesh and polyurethane coating on the effect of bone augmentation after titanium mesh exposure

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