聚醚酰亚胺为载体的Ce-Ag-ZnO复合涂层改性PEEK成骨及抗菌性能的研究

doi:10.12337/zgkqzzxzz.2025.02.010

中国口腔种植学杂志 ›› 2025, Vol. 30 ›› Issue (1): 54-61.DOI: 10.12337/zgkqzzxzz.2025.02.010

聚醚酰亚胺为载体的Ce-Ag-ZnO复合涂层改性PEEK成骨及抗菌性能的研究

戴雅婷^1,2, 韩泽奎^1,2, 汝晓娜^1,2, 吕玉光³, 李冰², 高士军^1,2

¹佳木斯大学口腔医学院佳木斯大学口腔医学工程实验中心黑龙江省生物医学材料及临床应用重点实验室 154002;
²佳木斯大学 154007,黑龙江;
³佳木斯大学药学院 154007,黑龙江

收稿日期:2024-09-02 出版日期:2025-02-28 发布日期:2025-02-24
通讯作者: 高士军,Email：gaoshijun822@163.com,电话：0454-8625531
作者简介:戴雅婷,硕士研究生,研究方向：口腔种植修复材料;高士军,修复科主任、主任医师、硕士研究生导师,研究方向：修复材料性能
基金资助:
2023年佳木斯大学教育教学改革研究项目（2023JY4-30）

Study on the osteogenic and antimicrobial properties of PEEK modified with a Ce-Ag-ZnO composite coating using PEI as a carrier

Dai Yating^1,2, Han Zekui^1,2, Ru Xiaona^1,2, Lyu Yuguang³, Li Bing², Gao Shijun^1,2

¹Stomatology Collage of Jiamusi University, Experimental Center for Stomatological Engineering, Jiamusi University, Key Laboratory of Oral Biomedical Materials and Clinical Application, Heilongjiang Province, Jiamusi 154002, Heilongjiang, China;
²Jiamusi University, Jiamusi 154007, Heilongjiang, China;
³School of Pharmacy, Jiamusi University, Jiamusi 154007, Heilongjiang, China

Received:2024-09-02 Online:2025-02-28 Published:2025-02-24
Contact: Gao Shijun, Email: gaoshijun822@163.com, Tel: 0086-454-8625531
Supported by:
2023 Jiamusi University Education and Teaching Reform Research Project(2023JY4-30)

摘要/Abstract

摘要： 目的以聚醚酰亚胺（polyetherimide,PEI）为载体涂覆铈-银-氧化锌（Ce-Ag-ZnO）复合涂层,改善聚醚醚酮（polyetheretherketone,PEEK）表面的成骨及抗菌性能。方法将Ce-Ag-ZnO复合粉末溶于PEI溶液,通过旋涂法将其涂于PEEK上形成均匀且牢固的涂层。实验分为空白组（PEEK）、对照组（PEEK+PEI）和实验组（PEEK+PEI+复合粉末）。分析试件表面形貌、亲水性、物相组成、实验组元素释放量及涂层附着力;采用CCK-8、活死细胞染色、鬼笔环肽染色、碱性磷酸酶相对酶活性测试、茜素红染色及实时定量PCR等实验分析试样成骨能力;平板菌落培养计数分析试样抑菌能力。结果 Ce-Ag-ZnO复合粉末成功涂于PEEK表面,分布较为均匀,且粉末可游离释放,实验组水接触角降低（P<0.001）;第7天实验组可见明显促进MC3T3-E1细胞增殖（P<0.001）,细胞黏附伪足伸展且清晰;茜素红染色矿化明显;改性后的PEEK可显著增强碱性磷酸酶（alkaline phosphatase,ALP）的合成（P<0.001）以及成骨基因Runx2、骨形态发生蛋白-2（bone morphogenetic protein-2,BMP-2）、骨桥蛋白（osteopontin,OPN）、骨钙素（osteocalcin,OCN）的表达（P<0.001）;实验组表面细菌量最少（P<0.001）。结论在PEEK表面制备复合涂层可以使材料的生物相容性、成骨、抗菌性更理想,优化了PEEK种植体的表面性能。

关键词: 成骨分化, 抗菌性能, 聚醚醚酮, 聚醚酰亚胺涂层, 稀土铈

Abstract: Objective This study aimed to utilize polyetherimide (PEI) as a carrier to coat a Ce-Ag-ZnO composite layer, enhancing the osteogenic and antibacterial properties of polyetheretherketone (PEEK). Methods Ce-Ag-ZnO composite powder was dissolved in a PEI solution and applied to PEEK via spin coating to form a uniform and stable coating. Three groups were established: the blank group (PEEK), the control group (PEEK+PEI), and the experimental group (PEEK+PEI+composite powder). The surface morphology, hydrophilicity, phase composition, element release, and coating adhesion were analyzed. The osteogenic potential of the samples was assessed using CCK-8, live/dead cell staining, cytoskeletal staining, ALP activity quantification, alizarin red staining, and quantitative reverse transcription PCR. The antibacterial efficacy was evaluated through bacterial colony counting. Results The Ce-Ag-ZnO composite powder was successfully coated onto the PEEK surface with a uniform distribution, allowing for controlled release. The experimental group exhibited significantly enhanced hydrophilicity (P<0.001). On the 7th day, the experimental group showed superior MC3T3-E1 cell proliferation (P<0.001), with well-spread pseudopodia and clear adhesion. Alizarin red staining demonstrated pronounced mineralization. The modified PEEK significantly upregulated ALP activity (P<0.001) and the expression of osteogenic genes, including Runx2, BMP-2, OPN, and OCN (P<0.001). The experimental group also exhibited the lowest bacterial adherence (P<0.001). Conclusion The composite coating on the PEEK surface enhanced its biocompatibility, osteogenic potential, and antibacterial properties, thereby optimizing the surface performance of PEEK implants.

Key words: Osteogenic differentiation, Antibacterial properties, Polyetheretherketone (PEEK), Polyetherimide (PEI) coatings, Rare earth cerium

戴雅婷,韩泽奎,汝晓娜,等. 聚醚酰亚胺为载体的Ce-Ag-ZnO复合涂层改性PEEK成骨及抗菌性能的研究[J]. 中国口腔种植学杂志, 2025, 30(1): 54-61. DOI: 10.12337/zgkqzzxzz.2025.02.010

Dai Yating, Han Zekui, Ru Xiaona, Lyu Yuguang, Li Bing, Gao Shijun. Study on the osteogenic and antimicrobial properties of PEEK modified with a Ce-Ag-ZnO composite coating using PEI as a carrier[J].Chinese Journal of Oral Implantology, 2025, 30(1): 54-61.DOI: 10.12337/zgkqzzxzz.2025.02.010.

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聚醚酰亚胺为载体的Ce-Ag-ZnO复合涂层改性PEEK成骨及抗菌性能的研究

Study on the osteogenic and antimicrobial properties of PEEK modified with a Ce-Ag-ZnO composite coating using PEI as a carrier

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