p53/CXCL12 在亲水性钛表面微纳米形貌促进人牙周膜成纤维细胞成骨分化中的作用

doi:10.12337/zgkqzzxzz.2019.12.002

中国口腔种植学杂志 ›› 2019, Vol. 24 ›› Issue (4): 154-157.DOI: 10.12337/zgkqzzxzz.2019.12.002

p53/CXCL12 在亲水性钛表面微纳米形貌促进人牙周膜成纤维细胞成骨分化中的作用

林玉娇, 庄秀妹, 谢奕文

515031 广东汕头汕头市中心医院口腔科(林玉娇, 谢奕文);广东广州中山大学附属孙逸仙纪念医院口腔科(庄秀妹)

出版日期:2019-12-10 发布日期:2021-09-07
通讯作者: 庄秀妹
基金资助:
国家自然科学基金青年基金项目(81600899)

Effect of hydrophilicity of titanium with micro/nanotopographical surface on osteogenic differentiation of human periodontal ligament cells via p53/CXCL12

LIN Yujiao, ZHUANG Xiumei, XIE Yiwen

Department of Stomatology, Shantou Central Hospital, Shantou 515031, Guangdong Province, China

Online:2019-12-10 Published:2021-09-07

摘要/Abstract

摘要： 目的: 探讨p53/CXCL12在亲水性钛表面微纳米形貌促进人牙周膜成纤维细胞(periodontal ligament cells,PDLCs)成骨分化中的作用。方法: 前期已采用阳极氧化法和喷砂碱热法构建相似微纳米形貌但亲水性不同钛表面,两组钛表面接种PDLCs,第7天时Western免疫印迹检测p53、CXCL12表达水平; 小干扰RNA(Si-p53、Si-CXCL12)转染PDLCs,检测分别敲低其表达后p53、CXCL12表达水平变化,比较碱性磷酸酶(ALP)活性,成骨标志物ALP、I型胶原(COL1)、成骨特异性转录因子(RUNX2)mRNA水平变化。采用SPSS13.0软件包对数据进行统计学分析。结果: 相比阳极氧化组,亲水性更优喷砂碱热组PDLCs中p53表达降低,CXCL12表达升高。Sip53转染PDLCs后,p53表达降低,CXCL12表达升高。Si-CXCL12转染PDLCs,CXCL12水平降低,p53水平无明显变化,但ALP、COL1与RUNX2的mRNA水平均显著升高。结论: 亲水性钛表面微纳米形貌可下调p53,促进CXCL12表达升高,进而促进PDLCs成骨分化。

关键词: 亲水性, 人牙周膜成纤维细胞, p53, CXCL12, 成骨分化, 微纳米形貌

Abstract: Objective: To investigate the effect of p53/CXCL12 on the osteogenic differentiation of periodontal ligament cells(PDLCs) induced by hydrophilicity of titanium with micro/nanotopographical surface. Methods: Anodic oxidation and sandblast-alkali heat methods were used to establish scale structures, which were cultured with PDLCs. Expressions of p53 and CXCL12 were examined by western blot at 7 day. After transfection of p53 or CXCL12 with siRNA, p53 and CXCL12 level were furthered detected, changes of alkaline phosphatase(ALP) activity and mRNA expressions of such osteogenic markers as ALP, collagen- I(COL1) and runt related transcription factor 2(RUNX2) were examined to detect osteogenic differentiation of PDLCs. Date was statistically analyzed with SPSS13.0 software package. Results: Compared with anodic oxidation surfaces, p53 expression of PDLCs on sandblast-alkali heat surface discs with well hydrophilicity was significantly decreased, while CXCL12 level increased. Transfected with Si-p53, p53 level was decreased and CXCL12 level increased. After transfection of Si-CXCL12, CXCL12 level was decreased, but no significant change of p53 level. However, ALP activity and ALP, COL1 and RUNX2 mRNA levels were significantly decreased. Conclusion: Hydrophilicity of titanium with micro/nanotopographical surface promotes osteogenic differentiation of PDLCs via p53/CXCL12.

Key words: hydrophilicity, periodontal ligament cells, p53, CXCL12, osteogenic differentiation, micro- nanotopographical surface

中图分类号:

R782

林玉娇,等庄秀妹,等谢奕文. p53/CXCL12 在亲水性钛表面微纳米形貌促进人牙周膜成纤维细胞成骨分化中的作用[J]. 中国口腔种植学杂志, 2019, 24(4): 154-157. DOI: 10.12337/zgkqzzxzz.2019.12.002

LIN Yujiao, ZHUANG Xiumei, XIE Yiwen. Effect of hydrophilicity of titanium with micro/nanotopographical surface on osteogenic differentiation of human periodontal ligament cells via p53/CXCL12[J].Chinese Journal of Oral Implantology, 2019, 24(4): 154-157.DOI: 10.12337/zgkqzzxzz.2019.12.002.

参考文献

[1] 庞静雯,吴亚霖,徐婷,等. 低氧激活HIF-1α抑制人牙周膜成纤维细胞的成骨分化[J]. 口腔疾病防治, 2017,(08): 488-493
[2] 周敏,武东辉,吴亚霖,等. 亲水性钛表面微纳米形貌对大鼠骨髓间充质干细胞增殖与成骨分化的影响[J]. 中华临床医师杂志(电子版), 2018,(02): 98-102
[3] 林志立,梁建平,庄秀妹. 亲水性钛表面微纳米形貌对人牙周膜成纤维细胞增殖与成骨分化的影响[J]. 中国口腔种植学杂志, 2018, 23(03): 101-104
[4] 周敏,吴亚霖,武东辉,等.钛表面微纳米形貌通过p53诱导大鼠骨髓间充质干细胞成骨分化的研究[J]. 中国口腔种植学杂志, 2017(03): 101-104
[5] 张叶,庄秀妹,张越,等. p53在低氧抑制人牙周膜成纤维细胞成骨分化中的作用[J]. 中华临床医师杂志(电子版), 2018, 12(09): 518-524
[6] Li Z, Wang W, Xu H, et al.Effects of altered CXCL12/ CXCR4 axis on BMP2/Smad/Runx2/Osterix axis and osteogenic gene expressions during osteogenic differentiation of MSCs[J]. Am J Transl Res, 2017, 9(4): 1680-1693
[7] Zhuang X M, Zhou B, Yuan K F.Role of p53 mediated miR-23a / CXCL12 pathway in osteogenic differentiation of bone mesenchymal stem cells on nanostructured titanium surfaces[J]. Biomed Pharmacother, 2019, 112 : 108649
[8] Zhuang X M, Zhou B, Ouyang J L, et al.Enhanced MC3T3-E1 preosteoblast response and bone formation on the addition of nano-needle and nano-porous features to microtopographical titanium surfaces[J]. Biomed Mater, 2014, 9(4):45001
[9] Lotz E M, Olivares-Navarrete R, Hyzy S L, et al.Comparable responses of osteoblast lineage cells to microstructured hydrophilic titanium-zirconium and microstructured hydrophilic titanium[J]. Clin Oral Implants Res, 2016.
[10] Hong Y S, Kim M J, Han J S, et al.Effects of hydrophilicity and fluoride surface modifications to titanium dental implants on early osseointegration: an in vivo study[J]. Implant Dent, 2014, 23(5): 529-533
[11] 王勤,庄秀妹,何杰玉,等. p53在低氧调控人牙周膜成纤维细胞增殖与凋亡中的作用[J]. 中华老年口腔医学杂志, 2019, 17(02): 70-74
[12] Winning L, Robinson L, Boyd A R, et al.Osteoblastic differentiation of per15iodontal ligament stem cells on nonstoichiometric calcium phosphate and titanium surfaces[J]. J Biomed Mater Res A, 2017, 105(6): 1692-1702
[13] Wang X, Kua H Y, Hu Y, et al.p53 functions as a negative regulator of osteoblastogenesis, osteoblast-dependent osteoclastogenesis, and bone remodeling[J]. J Cell Biol, 2006, 172(1): 115-125
[14] He Y, de Castro L F, Shin M H, et al. p53 loss increases the osteogenic differentiation of bone marrow stromal cells[J]. Stem Cells, 2015, 33(4): 1304-1319
[15] Liu C, Weng Y, Yuan T, et al.CXCL12/CXCR4 signal axis plays an important role in mediating bone morphogenetic protein 9-induced osteogenic differentiation of mesenchymal stem cells[J]. Int J Med Sci, 2013, 10(9): 1181-1192
[16] Arabanian L S, Fierro F A, Stolzel F, et al.MicroRNA- 23a mediates post-transcriptional regulation of CXCL12 in bone marrow stromal cells[J]. Haematologica, 2014, 99(6):997-1005

p53/CXCL12 在亲水性钛表面微纳米形貌促进人牙周膜成纤维细胞成骨分化中的作用

Effect of hydrophilicity of titanium with micro/nanotopographical surface on osteogenic differentiation of human periodontal ligament cells via p53/CXCL12

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