A-PRF对人骨髓间充质细胞生物学性能的影响

doi:10.12337/zgkqzzxzz.2021.10.003

中国口腔种植学杂志 ›› 2021, Vol. 26 ›› Issue (5): 282-288.DOI: 10.12337/zgkqzzxzz.2021.10.003

A-PRF对人骨髓间充质细胞生物学性能的影响

王佳, 赵瑞敏, 龚佳明, 余占海, 殷丽华

兰州大学口腔医学院（口腔医院）口腔种植科,甘肃 730000

收稿日期:2021-08-19 出版日期:2021-10-30 发布日期:2021-11-12
通讯作者: 殷丽华,Email: yinlh@lzu.edu.cn,电话：0931-8915062 王佳,硕士、医师,研究,方向：口腔种植学;殷丽华,教授、博士研究生,导师,研究方向：口腔种植学
作者简介:王佳,硕士、医师,研究,方向：口腔种植学；殷丽华,教授、博士研究生,导师,研究方向：口腔种植学
基金资助:
甘肃省省级引导科技创新发展竞争性项目专项资金（2018ZX-10）; 甘肃省科技计划国际合作专项（18YF1WA116）; 兰州市人才创新创业项目（2017-RC-31）

Effect of A-PRF on the biological performance of human bone marrow mesenchymal cells

Wang Jia, Zhao Ruimin, Gong Jiaming, Yu Zhanhai, Yin Lihua

Department of Oral Implant, School/Hospital of Stomatology, Lanzhou University, Lanzhou 730000, China

Received:2021-08-19 Online:2021-10-30 Published:2021-11-12
Contact: Yin Lihua, Email: yinlh@lzu.edu.cn, Tel: 0086-931-8915062

摘要/Abstract

摘要： 目的观察改良型富血小板纤维蛋白（advanced- platelet-rich fibrin, A-PRF）对人骨髓间充质细胞（human bone marrow mesenchymal cells, hBMMCs）生物学性能的影响。方法对hBMMCs进行多向分化能力鉴定,扫描电镜及人生长因子阵列膜检测A-PRF微观结构及分子构成,CCK-8检测A-PRF对hBMMCs增殖的影响,划痕实验检测A-PRF对hBMMCs迁移的影响,通过碱性磷酸酶活性检测及茜素红染色检测不同浓度A-PRF对hBMMCs矿化能力的影响。结果扫描电镜结果显示A-PRF为三维网状结构;人生长因子阵列膜检测发现A-PRF含有40多种细胞因子,并含有巨噬细胞集落刺激因子受体（macrophage colony stimulating factor receptor, M-CSF-R）及人神经生长因子β（β-nerve growth factor, β-NGF）;体外实验可见10% A-PRF可以提高细胞增殖（P<0.05）和迁移能力,同时可促进hBMMCs形成钙沉积和碱性磷酸酶活性表达（P<0.05）。结论 A-PRF对hBMMCs的成骨分化、增殖和迁移有促进作用,其中10%的A-PRF促进作用更为明显。因此,A-PRF对hBMMCs具有良好的细胞生物学效应,可为临床选择A-PRF促进骨组织再生提供初步实验依据。

关键词: A-PRF, 人骨髓间充质干细胞, 成骨分化

Abstract: Objective The aim of this study was to investigate the cytobiological effects of advanced-platelet rich fibrin (A-PRF) on cells from human bone marrow mesenchyme (hBMMCs) in vitro. Methods hBMMCs were identified as adipogenic and osteogenic differentiation. A-PRF was observed under a scanning electron microscope and its growth factor was detected by human growth factor antibody array-membrane. CCK-8 and scratch tests were used to detect the effect of A-PRF on the proliferation and migration of hBMMCs at different concentrations. Alkaline phosphatase activity and alizarin red staining were used to detect the effect of different concentrations of A-PRF on the mineralization ability of hBMMCs. Results SEM showed that A-PRF is a three-dimensional network containing more than 40 cytokines, among which macrophage colony stimulating factor and human nerve growth factor had not been reported. 10% A-PRF increased cell proliferation and migration ability (P<0.05), but also promoted calcium deposition and ALP of hBMMCs (P<0.05). Conclusion A-PRF improved the osteogenic differentiation of hBMMCs and the proliferation and migration, and 10% A-PRF achieved the most obvious promotion effect. The favorable cytobiological effects of A-PRF on hBMMCs might serve as the basis for A-PRF applications in bone regeneration.

Key words: Advanced-platelet rich fibrin, Human bone marrow mesenchymal cells, Osteogenic differentiation

王佳,赵瑞敏,龚佳明,等. A-PRF对人骨髓间充质细胞生物学性能的影响[J]. 中国口腔种植学杂志, 2021, 26(5): 282-288. DOI: 10.12337/zgkqzzxzz.2021.10.003

Wang Jia, Zhao Ruimin, Gong Jiaming, Yu Zhanhai, Yin Lihua. Effect of A-PRF on the biological performance of human bone marrow mesenchymal cells[J].Chinese Journal of Oral Implantology, 2021, 26(5): 282-288.DOI: 10.12337/zgkqzzxzz.2021.10.003.

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A-PRF对人骨髓间充质细胞生物学性能的影响

Effect of A-PRF on the biological performance of human bone marrow mesenchymal cells

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