不同钛表面形貌诱导人牙周膜成纤维细胞增殖与成骨分化的影响

doi:10.12337/zgkqzzxzz.2018.09.003

中国口腔种植学杂志 ›› 2018, Vol. 23 ›› Issue (3): 109-113.DOI: 10.12337/zgkqzzxzz.2018.09.003

不同钛表面形貌诱导人牙周膜成纤维细胞增殖与成骨分化的影响

刘根, 庄秀妹, 傅润英, 薛伟伟, 韦伟

518081 广东深圳深圳市第七人民医院口腔科（刘根, 傅润英, 薛伟伟, 韦伟）;广东广州中山大学附属孙逸仙纪念医院口腔科（庄秀妹）

出版日期:2018-09-10 发布日期:2021-09-06
通讯作者: 庄秀妹
基金资助:
国家自然科学基金青年基金项目（81600899）

Role of different scale structures of titanium surface on the proliferation and osteogenic differentiation of human periodontal ligament cells

LIU Gen, ZHUANG Xiumei, HU Renying, et al

Department of Stomatology, Shenzhen Seventh People's Hospital, Shenzhen 518081, Guangdong Province, China

Online:2018-09-10 Published:2021-09-06

摘要/Abstract

摘要： 目的： 比较不同钛表面形貌对人牙周膜成纤维细胞(periodontal ligament cells, PDLCs)增殖与成骨分化的影响。方法： 前期已构建不同形貌钛表面,包括喷砂碱热高温组的纳米针状表面、喷砂碱热低温组的纳米网状表面、喷砂酸蚀的微米凹坑表面和对照组的光滑纯钛表面。各组钛片表面接种PDLCs,在培养7天后采用MTT检测各组PDLCs增殖与总蛋白浓度,并进一步检测碱性磷酸酶（alkaline phosphatase, ALP）活性,同时采用实时荧光定量PCR分析成骨相关基因ALP、I型胶原（collagen-I,COL1）与成骨特异性转录因子（runt related transcriptionfactor 2, RUNX2）的mRNA表达量差异。采用SPSS13.0软件包对数据进行统计学分析。结果： 相比光滑纯钛组与喷砂酸蚀组,具有微纳米表面形貌的喷砂碱热高温组与喷砂碱热低温组PDLC细胞增殖能力显著增强,总蛋白浓度升高,ALP活性增加,ALP、COL1与RUNX2的mRNA表达水平明显上调。结论： 钛表面微纳米形貌可促进PDLC增殖与成骨分化。

关键词: 人牙周膜成纤维细胞, 钛表面形貌, 成骨分化, 微纳米形貌

Abstract: Objective: To investigate the effect of titanium surface scale structures on the cell viability and os-teogenic differentiation in periodontal ligament cells(PDLCs). Methods: Alkali heat treatment at high and low temperatures were utilized to nano-modify sandblasted titanium with microtopographical surfac-es, compared with microtopographical surfaces of conventional sandblast-acid etching and smooth surface in titanium control. PDLCs were seeding on these four titanium discs, and the cell viability, total protein values and alkaline phosphatase(ALP) activity were examined at 7 days. qRT-PCR was used to detect mRNA expression of ALP, collagen-I (COL1) and runt related transcription factor 2 (RUNX2) at 7 days. The date was statistically analyzed with SPSS13.0 software package. Results: Compared with Ti control and sandblast-acid etching surfaces, PDLCs on the micro-nanotopographical surface discs in alkali heat treatment at high temperatures group and low temperatures group exhibited enhanced cell viability, in-creased total protein values, up-regulated ALP activity and overexpression of ALP, COL1 and RUNX2 levels. Conclusion: Micro-nanotopographical surface of titanium implant promotes cell viability and os-teogenic differentiation of PDLCs.

Key words: human periodontal ligament cells, scale structures of titanium surface, osteogenic differ-entiation, micro-nanotopographical surface

中图分类号:

R783.4

刘根,庄秀妹,傅润英,等. 不同钛表面形貌诱导人牙周膜成纤维细胞增殖与成骨分化的影响[J]. 中国口腔种植学杂志, 2018, 23(3): 109-113. DOI: 10.12337/zgkqzzxzz.2018.09.003

LIU Gen, ZHUANG Xiumei, HU Renying, et al. Role of different scale structures of titanium surface on the proliferation and osteogenic differentiation of human periodontal ligament cells[J].Chinese Journal of Oral Implantology, 2018, 23(3): 109-113.DOI: 10.12337/zgkqzzxzz.2018.09.003.

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不同钛表面形貌诱导人牙周膜成纤维细胞增殖与成骨分化的影响

Role of different scale structures of titanium surface on the proliferation and osteogenic differentiation of human periodontal ligament cells

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