高压氧对高糖状态下成骨细胞增殖和分化的影响

doi:10.12337/zgkqzzxzz.2018.09.002

摘要/Abstract

摘要： 目的： 观察高压氧治疗对高糖状态下MC3T3-E1细胞增殖和成骨分化的影响。方法： 根据细胞培养基的葡萄糖浓度以及是否进行高压氧处理将细胞随机分为四组：1)5.5mM正常浓度组; 2)25.5mM高糖组; 3)5.5mM+高压氧组; 4)25.5mM+高压氧组。甲基噻唑基四唑（MTT）法测定1天,3天的细胞增殖;碱性磷酸酶（ALP）活性检测3天,7天的细胞分化情况;荧光实时定量PCR测定Run mRNA和OCN mRNA基因分别在3天、7天的表达。结果： 25.5mM高糖组的MC3T3-E1细胞的增殖、ALP活性以及成骨基因表达均显著低于5.5mM正常浓度组（P< 0.05）; 25.5mM+高压氧组的MC3T3-E1细胞的增殖及成骨分化较25.5mM高糖组显著提高（P< 0.05）。结论： 高压氧能显著改善高糖对MC3T3-E1细胞的增殖及成骨分化的抑制作用。

关键词: 高压氧, 糖尿病, 成骨细胞, 种植体, 骨结合

Abstract: Objectives: The aim of this project is to investigate the effect of hyperbaric oxygen therapy (HPO) on the proliferation and osteogenic ability of MC3T3-E1 osteoblastic cell line under high glucose condition. Methods: The cultures were divided into four treatment groups: 1) physiological medium with 5.5mM glu-cose, 2) high glucose medium with 25.5mM glucose, 3) 5.5mM glucose + HPO, 4) 25.5mM + HPO. At dif-ferent time point, the cell proliferation, alkaline phosphatase, and mineralized nodule, osteogenic gene ex-pression were detected. The software program SPSS was used to analyze all data, values of p<0.05 was con-sidered significant. Results: 25.5mM glucose concentrations inhibit the proliferation,ALP activity, miner-alization and osteogenic genes of MC3T3-E1 cells. Furthermore, the proliferation and osteogenic ability of MC3T3-E1 cells were significantly improved after hyperbaric oxygen therapy. Conclusions: Hyperbaric oxygen therapy promotes the proliferation and osteogenic ability of MC3T3-E1 cells under high glucose.

Key words: hyperbaric oxygen, diabetes, osteoblasts, implant, osseointegration

中图分类号:

782.13

董凯,郝鹏杰,周文娟,等. 高压氧对高糖状态下成骨细胞增殖和分化的影响[J]. 中国口腔种植学杂志, 2018, 23(3): 105-108. DOI: 10.12337/zgkqzzxzz.2018.09.002

DONG Kai, HAO Pengjie, ZHOU Wenjuan, et al. The influences of hyperbaric oxygen therapy on the proliferation and osteogenic differentiation of osteoblasts under high glucose condition[J].Chinese Journal of Oral Implantology, 2018, 23(3): 105-108.DOI: 10.12337/zgkqzzxzz.2018.09.002.

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