Animal experiment of prognosis of immediate implant placement and restoration in individuals with T2DM

doi:10.12337/zgkqzzxzz.2024.08.004

Abstract

Abstract: Objective To investigate the feasibility and prognosis of immediate implant placement and immediate restoration in patients with type 2 diabetes by establishing an animal model. Methods The type 2 diabetic animal model was established using New Zealand rabbits as the experimental group and healthy New Zealand rabbits as the control group. Both groups underwent immediate implantation and immediate repair. Micro-CT scanning, hard tissue section, and detection of relative inflammatory factors by real-time quantitative PCR were used to compare prognosis and histological remodeling between the two groups. Results The success rate for immediate implant placement and immediate restoration was 90% in both groups. In the early postoperative period (1~2 months), the peri-implant bone of the experimental rabbits in the T2DM group was significantly different from the control group in various microscopic parameters, in the meantime, the expressions of IL-6, IL-33 and TNF-α were significantly higher than those of the control group. Conclusion Animal experiments have shown that the immediate implant placement and immediate prosthesis are safe in type 2 diabetic animal models. However, attention should be paid to the slow formation of osseointegration and obvious inflammation of the soft tissue around the implant in the early stage.

Key words: Dental implant, Immediate implant placement, Immediate restoration, Animal experiment, Type 2 diabetes mellitus

Zhao Guoqiang, Song Yingliang. Animal experiment of prognosis of immediate implant placement and restoration in individuals with T2DM[J]. Chinese Journal of Oral Implantology, 2024, 29(4): 311-319.

References

[1] Alasqah M, Mokeem S, Alrahlah A, et al.Periodontal parameters in prediabetes, type 2 diabetes mellitus, and non-diabetic patients[J]. Braz Oral Res, 2018,32:e81. DOI: 10.1590/1807-3107bor-2018.vol32.0081.
[2] Raju K, Taylor GW, Tahir P, et al.Association of tooth loss with morbidity and mortality by diabetes status in older adults: a systematic review[J]. BMC Endocr Disord, 2021,21(1):205. DOI: 10.1186/s12902-021-00830-6.
[3] Power DJ, Sambrook PJ, Goss AN.The healing of dental extraction sockets in insulin-dependent diabetic patients: a prospective controlled observational study[J]. Aust Dent J, 2019,64(1):111-116. DOI: 10.1111/adj.12669.
[4] Wang F, Song YL, Li DH, et al.Type 2 diabetes mellitus impairs bone healing of dental implants in GK rats[J]. Diabetes Res Clin Pract, 2010,88(1):e7-e9. DOI: 10.1016/j.diabres.2010.01.017.
[5] 陆添, 吴蔚, 刘敏. Ⅱ型糖尿病患者种植牙二期手术后牙龈愈合状况研究[J].健康研究,2017,37(4):417-419,423. DOI: 10.3969/j.issn.1674-6449.2017.04.017.
[6] Arora H, Ivanovski S.Clinical and aesthetic outcomes of immediately placed single-tooth implants with immediate vs. delayed restoration in the anterior maxilla: a retrospective cohort study[J]. Clin Oral Implants Res, 2018,29(3):346-352. DOI: 10.1111/clr.13125.
[7] Alqutaibi AY, Hussain BS, Alobodi AK.Immediate and conventional loading of a single dental implant placed in the esthetic zone may yield comparable clinical outcomes[J]. J Evid Based Dent Pract, 2020,20(4):101492. DOI: 10.1016/j.jebdp.2020.101492.
[8] Slagter KW, Raghoebar GM, Hentenaar D, et al.Immediate placement of single implants with or without immediate provisionalization in the maxillary aesthetic region: a 5-year comparative study[J]. J Clin Periodontol, 2021,48(2):272-283. DOI: 10.1111/jcpe.13398.
[9] Shi S, Song S, Liu X, et al.Construction and performance of exendin-4-loaded chitosan-PLGA microspheres for enhancing implant osseointegration in type 2 diabetic rats[J]. Drug Deliv, 2022,29(1):548-560. DOI: 10.1080/10717544.2022.2036873.
[10] Gadicherla S, Smriti K, Roy S, et al.Comparison of extraction socket healing in non-diabetic, prediabetic, and type 2 diabetic patients[J]. Clin Cosmet Investig Dent, 2020,12:291-296. DOI: 10.2147/CCIDE.S264196.
[11] Mitsias M, Siormpas K, Pistilli V, et al.Immediate, early (6 weeks) and delayed loading (3 months) of single, partial and full fixed implant supported prostheses: 1-year post-loading data from a multicentre randomised controlled trial[J]. Eur J Oral Implantol, 2018,11(1):63-75.
[12] Al Hezaimi K, Naghshbandi J, Nooh N, et al.Buccal bone remodeling around immediate implants in STZ-induced diabetic dogs: a histologic and microcomputed tomographic analysis[J]. Int J Periodontics Restorative Dent, 2021,41(5):683-690. DOI: 10.11607/prd.4589.
[13] Andrade C, Paz J, de Melo GS, et al. Survival rate and peri-implant evaluation of immediately loaded dental implants in individuals with type 2 diabetes mellitus: a systematic review and meta-analysis[J]. Clin Oral Investig, 2022,26(2):1797-1810. DOI: 10.1007/s00784-021-04154-6.
[14] Ibraheem EMA, Hammad HGH, El-Sisy AME.Comparing marginal bone height changes around immediately and delayed implant-retained mandibular overdentures in controlled diabetic patients: a randomized clinical study[J].Bull Natl Res Cent, 2019, 43(1):1-8. DOI: 10.1186/s42269-019-0228-1.
[15] 王宗保, 姚峰, 吴端生, 等. 高糖高脂致家兔糖尿病的实验模型探讨[J].中国实验动物学报,2003,11(2):103-106. DOI: 10.3969/j.issn.1005-4847.2003.02.010.
[16] Lagunov VL, Sun J, George R.Evaluation of biologic implant success parameters in type 2 diabetic glycemic control patients versus health patients: a meta-analysis[J]. J Investig Clin Dent, 2019,10(4):e12478. DOI: 10.1111/jicd.12478.
[17] Al-Shibani N, Al-Aali KA, Al-Hamdan RS, et al.Comparison of clinical peri-implant indices and crestal bone levels around narrow and regular diameter implants placed in diabetic and non-diabetic patients: a 3-year follow-up study[J]. Clin Implant Dent Relat Res, 2019,21(2):247-252. DOI: 10.1111/cid.12712.
[18] Power DJ, Sambrook PJ, Goss AN.The healing of dental extraction sockets in insulin-dependent diabetic patients: a prospective controlled observational study[J]. Aust Dent J, 2019,64(1):111-116. DOI: 10.1111/adj.12669.
[19] Wang F, Song YL, Li DH, et al.Type 2 diabetes mellitus impairs bone healing of dental implants in GK rats[J]. Diabetes Res Clin Pract, 2010,88(1):e7-e9. DOI: 10.1016/j.diabres.2010.01.017.
[20] Andrade C, Paz J, de Melo GS, et al. Survival rate and peri-implant evaluation of immediately loaded dental implants in individuals with type 2 diabetes mellitus: a systematic review and meta-analysis[J]. Clin Oral Investig, 2022,26(2):1797-1810. DOI: 10.1007/s00784-021-04154-6.
[21] Al Amri MD, Alfarraj Aldosari AM, Al-Johany SS, et al.Comparison of clinical and radiographic status around immediately loaded versus conventional loaded implants placed in patients with type 2 diabetes: 12- and 24-month follow-up results[J]. J Oral Rehabil, 2017,44(3):220-228. DOI: 10.1111/joor.12466.
[22] Al Amri MD, Kellesarian SV, Al-Kheraif AA, et al.Effect of oral hygiene maintenance on hba1c levels and peri-implant parameters around immediately-loaded dental implants placed in type-2 diabetic patients: 2 years follow-up[J]. Clin Oral Implants Res, 2016,27(11):1439-1443. DOI: 10.1111/clr.12758.
[23] Juncar RI, Precup AI, Juncar M.Immediate implant-prosthetic dental rehabilitation of patients with diabetes using four immediately loaded dental implants: a pilot study[J]. J Int Med Res, 2020,48(3):300060519897195. DOI: 10.1177/0300060519897195.
[24] 王力锋, 方开秀, 许晓茹, 等. 2型糖尿病大鼠颅骨极限骨缺损的实验研究[J].实用口腔医学杂志,2015,(2):157-161. DOI: 10.3969/j.issn.1001-3733.2015.02.02.
[25] Yang M, Xie J, Lei X, et al.Tubeimoside I suppresses diabetes-induced bone loss in rats, osteoclast formation, and rankl-induced nuclear factor-κB pathway[J]. Int Immunopharmacol, 2020,80:106202. DOI: 10.1016/j.intimp.2020.106202.
[26] Akram Z, Rahim ZH, Taiyeb-Ali TB, et al.Resistin as potential biomarker for chronic periodontitis: a systematic review and meta-analysis[J]. Arch Oral Biol, 2017,73:311-320. DOI: 10.1016/j.archoralbio.2016.08.016.
[27] Zhang Y, Jia R, Zhang Y, et al.Effect of non-surgical periodontal treatment on cytokines/adipocytokines levels among periodontitis patients with or without obesity: a systematic review and meta-analysis[J]. BMC Oral Health, 2023,23(1):717. DOI: 10.1186/s12903-023-03383-3.
[28] Sun R, Liang C, Sun Y, et al.Effects of metformin on the osteogenesis of alveolar BMSCs from diabetic patients and implant osseointegration in rats[J]. Oral Dis, 2022,28(4):1170-1180. DOI: 10.1111/odi.13808.
[29] Wang X, Jiang L, Shao X.Association analysis of insulin resistance and osteoporosis risk in chinese patients with T2DM[J]. Ther Clin Risk Manag, 2021,17:909-916. DOI: 10.2147/TCRM.S328510.
[30] King S, Klineberg I, Levinger I, et al.The effect of hyperglycaemia on osseointegration: a review of animal models of diabetes mellitus and titanium implant placement[J]. Arch Osteoporos, 2016,11(1):29. DOI: 10.1007/s11657-016-0284-1.
[31] Sam L, Chattipakorn S, Khongkhunthian P.Osseointegration of maxillary dental implants in diabetes mellitus patients: a randomized clinical trial human histomorphometric study[J].Appl Sci, 2020, 10(19):6762. DOI: 10.3390/app10196762.
[32] Yu T, Acharya A, Mattheos N, et al.Molecular mechanisms linking peri-implantitis and type 2 diabetes mellitus revealed by transcriptomic analysis[J]. PeerJ, 2019,7:e7124. DOI: 10.7717/peerj.7124.
[33] Guzeldemir E, Gunhan M, Ozcelik O, et al.Interleukin-1 and tumor necrosis factor-alpha gene polymorphisms in turkish patients with localized aggressive periodontitis[J]. J Oral Sci, 2008,50(2):151-159. DOI: 10.2334/josnusd.50.151.
[34] Mokeem S, Alfadda SA, Al-Shibani N, et al.Clinical and radiographic peri-implant variables around short dental implants in type 2 diabetic, prediabetic, and non-diabetic patients[J]. Clin Implant Dent Relat Res, 2019,21(1):60-65. DOI: 10.1111/cid.12691.