The influences of hyperbaric oxygen therapy on the proliferation and osteogenic differentiation of osteoblasts under high glucose condition

doi:10.12337/zgkqzzxzz.2018.09.002

Abstract

Abstract: Objectives: The aim of this project is to investigate the effect of hyperbaric oxygen therapy (HPO) on the proliferation and osteogenic ability of MC3T3-E1 osteoblastic cell line under high glucose condition. Methods: The cultures were divided into four treatment groups: 1) physiological medium with 5.5mM glu-cose, 2) high glucose medium with 25.5mM glucose, 3) 5.5mM glucose + HPO, 4) 25.5mM + HPO. At dif-ferent time point, the cell proliferation, alkaline phosphatase, and mineralized nodule, osteogenic gene ex-pression were detected. The software program SPSS was used to analyze all data, values of p<0.05 was con-sidered significant. Results: 25.5mM glucose concentrations inhibit the proliferation,ALP activity, miner-alization and osteogenic genes of MC3T3-E1 cells. Furthermore, the proliferation and osteogenic ability of MC3T3-E1 cells were significantly improved after hyperbaric oxygen therapy. Conclusions: Hyperbaric oxygen therapy promotes the proliferation and osteogenic ability of MC3T3-E1 cells under high glucose.

Key words: hyperbaric oxygen, diabetes, osteoblasts, implant, osseointegration

CLC Number:

782.13

DONG Kai, HAO Pengjie, ZHOU Wenjuan, et al. The influences of hyperbaric oxygen therapy on the proliferation and osteogenic differentiation of osteoblasts under high glucose condition[J]. Chinese Journal of Oral Implantology, 2018, 23(3): 105-108.

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