Table of Content

10 September 2018, Volume 23 Issue 3

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Hydrophilicity of titanium with micro/nanotopographical surface promotes proliferation and osteogenic differentiation of human periodontal ligament cells

LIN Zhili, LIANG Jianping, ZHUANG Xiumei

2018, 23(3):  101-104.  DOI: 10.12337/zgkqzzxzz.2018.09.001

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Objective: To investigate the effect of hydrophilicity of titanium with micro/nanotopographical sur-face on the cell viability and osteogenic differentiation in human periodontal ligament cells (PDLCs). Methods: Methods of anodic oxidation and sandblast-alkali heat were utilized to establish scale struc-tures. PDLCs were seeded on these two titanium discs, cell viability was detected at 1, 4, 7 and 14 days by CCK8 assay. Total protein values and alkaline phosphatase(ALP) activity were examined at 7 and 14 days. ALP, collagen-I(COL1) and runt related transcription factor 2(RUNX2) mRNA expression at 7 days were detected by qRT-PCR. The date was statistically analyzed with SPSS13.0 software package. Results: Compared with anodic oxidation surfaces, PDLCs on the sandblast-alkali heat surface discs with well hy-drophilicity exhibited enhanced cell viability, increased total protein values, up-regulated ALP activity and overexpression of ALP, COL1 and RUNX2 levels. Conclusion: Micro-nanotopographical surface of titanium implant in sandblast-alkali heat group promotes cell viability and osteogenic differentiation of PDLCs, which may be affected by its hydrophilicity.

The influences of hyperbaric oxygen therapy on the proliferation and osteogenic differentiation of osteoblasts under high glucose condition

DONG Kai, HAO Pengjie, ZHOU Wenjuan, et al

2018, 23(3):  105-108.  DOI: 10.12337/zgkqzzxzz.2018.09.002

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Objectives: The aim of this project is to investigate the effect of hyperbaric oxygen therapy (HPO) on the proliferation and osteogenic ability of MC3T3-E1 osteoblastic cell line under high glucose condition. Methods: The cultures were divided into four treatment groups: 1) physiological medium with 5.5mM glu-cose, 2) high glucose medium with 25.5mM glucose, 3) 5.5mM glucose + HPO, 4) 25.5mM + HPO. At dif-ferent time point, the cell proliferation, alkaline phosphatase, and mineralized nodule, osteogenic gene ex-pression were detected. The software program SPSS was used to analyze all data, values of p<0.05 was con-sidered significant. Results: 25.5mM glucose concentrations inhibit the proliferation,ALP activity, miner-alization and osteogenic genes of MC3T3-E1 cells. Furthermore, the proliferation and osteogenic ability of MC3T3-E1 cells were significantly improved after hyperbaric oxygen therapy. Conclusions: Hyperbaric oxygen therapy promotes the proliferation and osteogenic ability of MC3T3-E1 cells under high glucose.

Role of different scale structures of titanium surface on the proliferation and osteogenic differentiation of human periodontal ligament cells

LIU Gen, ZHUANG Xiumei, HU Renying, et al

2018, 23(3):  109-113.  DOI: 10.12337/zgkqzzxzz.2018.09.003

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Objective: To investigate the effect of titanium surface scale structures on the cell viability and os-teogenic differentiation in periodontal ligament cells(PDLCs). Methods: Alkali heat treatment at high and low temperatures were utilized to nano-modify sandblasted titanium with microtopographical surfac-es, compared with microtopographical surfaces of conventional sandblast-acid etching and smooth surface in titanium control. PDLCs were seeding on these four titanium discs, and the cell viability, total protein values and alkaline phosphatase(ALP) activity were examined at 7 days. qRT-PCR was used to detect mRNA expression of ALP, collagen-I (COL1) and runt related transcription factor 2 (RUNX2) at 7 days. The date was statistically analyzed with SPSS13.0 software package. Results: Compared with Ti control and sandblast-acid etching surfaces, PDLCs on the micro-nanotopographical surface discs in alkali heat treatment at high temperatures group and low temperatures group exhibited enhanced cell viability, in-creased total protein values, up-regulated ALP activity and overexpression of ALP, COL1 and RUNX2 levels. Conclusion: Micro-nanotopographical surface of titanium implant promotes cell viability and os-teogenic differentiation of PDLCs.

Experimental study on comparison of lifting effect between maxillary sinus in IBS and Summers osteotome

WEN Hongxia, FAN Yawei

2018, 23(3):  114-118.  DOI: 10.12337/zgkqzzxzz.2018.09.004

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Objective: To establish an experimental model of goat animal in the maxillary sinus and compare the effect of IBS osteotome and Summers osteotome on the maxillary sinus lift in goats. Methods: A large number of pre-experimental anatomical analysis of goat maxillary sinus, combined with goat CBCT to ex-plore the maxillary sinus lifting site, established an experimental model of goat animal lifted in the maxil-lary sinus. The 18 local goats with 36 maxillary sinus were divided into experimental group and control group. The experimental group used IBS osteotome. The control group used Summers osteotome. The 18 goats were randomly divided into 5, 8, 10mm according to the maxillary sinus lifting height. Three groups, each with 6 sheep heads, counted the number of perforations in each experimental group and control group and recorded the height of maxillary sinus lift during perforation. Results: An animal model was es-tablished for the maxillary sinus elevation in the 6-8mm lobe of the maxillary first molar of the goat. The number of perforation of the maxillary sinus was 0, 1, and 3 in each experimental group, and the number of perforation in the maxillary sinus was 1, 5, and 6, respectively. There was no significant difference in safety between the experimental group and the control group when the height was 5 mm in the maxillary sinus[(5.00±0.00) mm vs(4.92±0.20)mm, P>0.05]. The experimental group and the control group were in the maxillary sinus. When the height was 8mm, the difference was significant[(7.83±0.41)mm vs (6.58± 1.32)mm, P<0.05]. There was significant difference in safety between the experimental group and the con-trol group when the maxillary sinus height was 10mm. (9.17±1.57)mm vs(6.08±0.97) mm, P<0.05]. Conclusion: The maxillary sinus elevation of the maxillary first molar is significantly improved in the maxil-lary sinus. When the lifting height is less than 5mm, there is no significant difference between the two lift-ing effects. The lifting height is greater than 5mm. IBS osteotome has better improvement than Summers osteotome.

Tooth-and locking taper implant-supported prostheses: a retrospective clinical study

WANG Yi, CEN Wen, HUANG Jiangqin, et al

2018, 23(3):  119-123.  DOI: 10.12337/zgkqzzxzz.2018.09.005

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Objective: The purpose of this study was to evaluate clinical treatment outcomes of fixed partial den-ture with combination of locking taper implant and natural teeth as abutments, and to analyze relevant fac-tors. Methods: Since January 2013-January 2015, a total of 19 implants were placed in 11patients, 17 (89%) in the maxilla and 2(11%) in the mandible. The implants were connected to 23 abutment teeth, 20 in the maxilla and 3 in the mandible of the prosthetic restorations, 34 were porcelain-fused-to-metal, 10 were all ceramic crown. The observation period ranged from 1 year to 3 years recording the average annu-al marginal bone resorption, soft tissue around the implant, technical complications and patient satisfac-tion, and calculating the implant survival rate. Results: The implant cumulative rate for 3 years was 100%, and the average marginal bone resorption was(0.10±0.02)mm. During the following observation pe-riod, 1 patient with diabetes and periodontitis after postloading in 3 month, occurrencing mild gingival swelling, no significant spontaneous bleeding, no pain. About technical complications, 1 patient appeared the crown fall off, 1 patient appeared the porcelain shipping-off, the remaining patients no obvious abnor-malities. The satisfaction is high. Conclusion: The results from the present study support the findings that treatment that combines natural teeth and locking taper implants in the support of rigid superstruc-tures has excellent follow-up results. Nonetheless, results on this clinical study should be validated with a longer evidence.

Clinical efficacy and safety evaluation of minimally invasive implantation in elderly patients with dentition defects

GONG Botao, ZHANG Jianguo

2018, 23(3):  124-126.  DOI: 10.12337/zgkqzzxzz.2018.09.006

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Objective: To explore and analyze the clinical efficacy and safety of minimally invasive implanta-tion in elderly patients with dentition defects. Methods: 58 elderly patients with dental defect admitted from April 2013 to April 2018 were selected as the subjects in this study. They were divided into 29 cases in each group by random numerical tables. Among them, the experimental group performed minimally in-vasive planting techniques, and the control group performed conventional dental implants. Results: The effective rate of treatment was significantly better than that of control group（P<0.05）. The time of opera-tion and application of antibiotics were shorter in the experimental group than in the control group(P< 0.05). The incidence of complications in the test group was significantly better than that in the control group（P<0.05）. Patients in the test group were significantly more satisfied than those in the control group （P<0.05）. Conclusion: Minimally invasive implantation for elderly patients with dentition defects not only has a good therapeutic effect, but also can shorten the operation time, can also reduce the occurrence of complications, can improve the satisfaction of treatment, and has a better clinical efficacy and safety, worthy of promotion and application.

Effect of root reduction flap on reconstruction of buccal keratinized gingiva in dental implants

WANG Xinquan

2018, 23(3):  127-130.  DOI: 10.12337/zgkqzzxzz.2018.09.007

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Objective: To study the effect of root-reposition flap in reconstruction of buccal keratinized gingi-val. Methods: From June 2015 to June 2017, 100 patients with missing or too narrow buccal keratinized gingival were selected from our department of stomatology. According to the different methods of buccal keratinization gingival reconstruction were divided into control group and observation group with 50 cases in each group. Among them, the patients in the control group were treated with traditional implant meth-ods. In the observation group, the buccal keratinized gingiva was reconstructed by root reduction flap tech-nique. The reconstruction effect of buccal keratinized gingival was compared between the two groups. Results: There were 27 cases(54.00%) cured person in the observation group. The total effective rate of treat-ment was 94.00. Which were significantly higher than the control group 14 cases (P<0.05). After repair, the observation group of width of keratinized gingival was(1.85±0.71) mm, and the thickness of keratin-ized gingival was(2.91±0.53)mm, which was significantly better than the control group（1.23±0.33）mm, （1.98±0.46）mm(P<0.05). The gingival index of the implant teeth in the observation group was(0.38 ± 0.23) after 6 months. It was significantly lower than that in the control group (0.54 ±0.25)(P<0.05). There was no significant difference in gingival index, gingival bleeding between adjacent teeth and operation ar ea between the two groups. The degree of anastomosis of adjacent teeth, the beauty of crown and the de-gree of tooth imperfection in the observation group were significantly better than the control group(P< 0.05). Conclusion: The application of root reduction flap in the reconstruction of buccal keratinized gingi-val is more effective, good effect, high aesthetic degree and quick recovery, so it can be widely recom-mended in clinic.

Analysis of factors related to early failure after implant implantation

HUANG Lijuan, MU Yandong, QING Wei, et al

2018, 23(3):  131-135.  DOI: 10.12337/zgkqzzxzz.2018.09.008

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Objective: To analyze the factors related to the failure of implants in the department of stomatology in the past five years. Methods: A total of 392 cases were implanted in the Department of Oral Implantol-ogy of People's Hospital of Sichuan Province from January 2013 to December 2017. A total of 392 men were enrolled in this study, 212 of whom were males and 180 were females. A total of 598 implants were implanted. There were 116 Dentiums, 170 American Biohorizons, and 312 Straumanns from Switzerland. Table analysis was performed. Chi-square test was used to analyze the early failure of the implants in terms of gender, different implant systems, different bone augmentation procedures, different implant sites, and implant size. The relevant factors of the rate. Results: According to the analysis of case data, there were 8 cases of early failure within 5 years and 10 implants. The early failure rate of implants was 1.67%. In terms of gender, the early failure rate after implantation was not statistically significant(p>0.05), there was no significant difference in early failure rate between different implant systems(p>0.05), the ear-ly failure of maxillary sinus extraction and bone grafting. The rate was highest, but it was not statistically significant compared to other bone augmentation surgeries. The number of implanted mandibular anterior teeth was the lowest but the early failure rate was higher than other sites and statistically significant (p< 0.05), fine implants (Diameter≤3.8mm), the early failure rate of short implants(length≤10mm) was signifi-cantly higher than other types of implants, and there was a statistically significant(p<0.05). Conclusions: The early failure rate of implants in the anterior mandibular area, narrow implants and short implant placements are much higher.

Application effect and prognosis of bone graft and tissue engineering scaffold materials in maxillary sinus lifting oral implant restoration

JIANG Jian

2018, 23(3):  136-138.  DOI: 10.12337/zgkqzzxzz.2018.09.009

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Objective: To investigate the application effect and prognosis of bone graft and tissue engineering scaffold in maxillary sinus lifting oral implant restoration. Methods: Thirty patients with maxillary sinus and oral implants were selected. All of them were treated from May 2016 to May 2017. Bio-Oss collagen was applied as a scaffold for bone grafting and tissue engineering. The effect and prognosis were observed. Results: A total of 30 patients were selected in this study. A total of 34 threaded column implants were implanted. There was no infection and damage of the maxillary sinus mucosa and gingival mucosa. After 6 months of follow-up, X-ray examination and artificial bone fusion of the alveolar bone were ideal, and the implant was effectively combined with the surrounding bone. After an average of 24 months of follow-up, the denture can play a normal role. Conclusion: For patients with clinically treated maxillary sinus eleva-tion for oral implant restoration, Bio-Oss collagen is applied as a bone graft and tissue engineering scaf-fold material. The implant bone combination is ideal, which can avoid loosening and falling off. Satisfacto-ry repair results provide a strong guarantee.

Comparison of implant denture and fixed denture in oral restoratipn

CHEN Yu

2018, 23(3):  139-141.  DOI: 10.12337/zgkqzzxzz.2018.09.010

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Objective: To apply implant denture in the process of oral repair and analyze the value of clinical application. Methods: Select our hospital 128 cases of patients with the need for oral cavity repair as the research object, the selected time period from 2011 to 2011, using the method of retrospective type, ac-cording to different patients to choose way of repair were divided into control group (n=64) and planting group(n=64), control group patients with conventional fixed denture, planting planting denture group of pa-tients, to evaluate the repair effect of two groups of patients. Results: There was no significant difference in BI score between the two groups before treatment, and there was no statistical significance, P>0.05. Af-ter repair, the BI score of the implant group was significantly lower than that of the control group, and the advantage was obvious, P<0.05. The functional recovery time of the implant group was (5.01 + 1.14) months, significantly longer than that of the control group, P<0.05. The total effective rate of patients in both the implant group and the control group was higher than 90.00%, and there was no significant differ-ence, P>0.05. The patients in the implant group were 96.88% satisfied with the repair effect, which was significantly higher than the control group's 79.69%, P<0.05. Conclusion: Implantable denture repair can achieve similar results to fixed denture repair in oral repair, and it will not cause damage to healthy teeth. The satisfaction of patients is higher.

Statistical analysis of 973 cases (2224 domestic implant prostheses)

WANG Guang, REN Daopu, HE Lu, et al

2018, 23(3):  142-146.  DOI: 10.12337/zgkqzzxzz.2018.09.011

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Objective: To preliminarily evaluate the clinical effects of two kinds of domestic systems and meth-ods for bony union implant denture. Methods: From January 2003 to March 2014, 2224 homemade bonebonded implants were implanted in our department. They were 832 HBIC implants developed by Hebei Medical University and 1392 CDIC implants developed by the China dental implantology center(West Chi-na Medical University). Repair design: 938 cases of fixed repair, 35 cases of active repair, a total of 973 patients(including jaw defect repair and reconstruction cases). The longest implantation time was 134 months, the shortest implantation time was 52 months, and the average implantation time was 93 months. The methods of observation were X-ray examination and clinical examination. Results: 29 of the implants were loosened before the upper structure was restored, 52 were loosened after the upper structure was re-stored, and 2142 were retained. Conclusion: The 134 month survival rate of domestic implant is 96.31%.

Minimally invasive immediate implant restoration in maxillary aesthetic zone with multiple teeth loss: case report

DONG Xin, WANG Lijun, YANG Xiaodong

2018, 23(3):  147-150.  DOI: 10.12337/zgkqzzxzz.2018.09.012

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Objective: This study is aimed to investigate the clinical effect of minimally invasive immediate im-plant restoration in maxillary aesthetic zone with multiple teeth loss. Methods: This study did the immedi-ate implant surgery with bone expansion and bone augmentation technique in a minimally invasive flap-less way using 2 Nobel-Replace bone level implants. And the permanent restoration was performed 6 months after the implant surgery. The synosteosis and periprosthetic soft tissue were observed 3, 30, 72 months later. Results: The synosteosis was well formed and the impants were stable after long-term load-ed. And the periprosthetic soft tissue had healthy pink aesthetics and proper white aesthetics. The treat-ment effect was beyond expectation and the patient left with great satisfaction. Conclusion: The minimal-ly invasive immediate implant restoration can meet both the functional and aesthetic demand of the pa-tients with multiple teeth loss in maxillary aesthetic zone which is significant in clinical therapy to some degree.