复合万古霉素后对载有BMP2 的仿生磷酸钙骨替代材料成骨性能影响的研究

doi:10.12337/zgkqzzxzz.2019.06.001

中国口腔种植学杂志 ›› 2019, Vol. 24 ›› Issue (2): 51-54.DOI: 10.12337/zgkqzzxzz.2019.06.001

• 专题研究 • 下一篇

复合万古霉素后对载有BMP2 的仿生磷酸钙骨替代材料成骨性能影响的研究

王冬, 罗媛, 彭娟红

518035 广东深圳北京大学深圳医院口腔医学中心

出版日期:2019-06-10 发布日期:2021-09-07
通讯作者: 彭娟红
基金资助:
国家自然科学基金青年基金项目(31800808)

The influence of vancomycin on osteogenic efficacy of BMP2-coprecipated biomimetic calcium phosphate granules

WANG Dongyun, LUO Yuan, PENG Juanhong

Department of Stomatology, Beijing University Shenzhen Hospital,Shenzhen 518035, Guangdong Province, China

Online:2019-06-10 Published:2021-09-07

摘要/Abstract

摘要： 目的： 探讨复合万古霉素后对载有BMP2的仿生磷酸钙骨替代材料(BMP2-coprecipated biomimetic calci-um phosphate granules,BioCaP-BMP2)成骨性能的影响。方法： 在BioCaP-BMP2上接种BMSCs,观察不同浓度(0,1, 10,30μg/ml)万古霉素对细胞生物学行为的影响。采用CCK8法在培养1、4、7天时检测BMSCs的增殖; 第4、7天检测总蛋白浓度与碱性磷酸酶活性及骨钙素的表达。结果： 相比对照组,1μg/ml、10μg/ml组中BMSCs表现出与对照组相当的增殖和成骨分化能力,而30μg/ml组万古霉素显示对BMSCs的抑制作用。结论： 治疗浓度的万古霉素不会影响BioCaP-BMP2的成骨效果,构建复合万古霉素的BioCaP-BMP2的颗粒有望成为治疗感染性骨缺损的理想材料。;

关键词: 骨髓间充质干细胞, 万古霉素, 骨形态发生蛋白-2, 成骨分化

Abstract: Objective： To investigate the influence of vancomycin on osteogenic efficacy of BMP2-coprecipated biomimetic calcium phosphate granules. Methods： After being seeded on BioCaP-BMP2 granules, BM- SCs were treated with vancomycin at concentration of 0,1, 10, 30μg/ml. Cell proliferation was detected at 1, 4, 7 days by CCk8 assay. Total protein, alkaline phosphatase activity and osteocalcin expression were examined at 4 and 7 days. Results： Compared with control group, vancomycin at concentration of 1 and 10μg/ml did not show negative effects on the biological behavior of BMSCs. On the contrary, high concen- tration of vancomycin was found to inhibit the proliferation and osteogenic differentiation of BMSCs. Conclusion: Therapeutic concentration of vancomycin will not negatively influence the osteogenic efficacy of BioCaP-BMP2, which means BioCaP-BMP2 granules carrying vancomycin could be a promising material to treat infectious bone defects.

Key words: bone marrow mesenchymal stem cells, vancomycin, bone morphogenetic protein 2, os- teogenic differentiation

中图分类号:

783.1

王冬,等罗媛,等彭娟红. 复合万古霉素后对载有BMP2 的仿生磷酸钙骨替代材料成骨性能影响的研究[J]. 中国口腔种植学杂志, 2019, 24(2): 51-54. DOI: 10.12337/zgkqzzxzz.2019.06.001

WANG Dongyun, LUO Yuan, PENG Juanhong. The influence of vancomycin on osteogenic efficacy of BMP2-coprecipated biomimetic calcium phosphate granules[J].Chinese Journal of Oral Implantology, 2019, 24(2): 51-54.DOI: 10.12337/zgkqzzxzz.2019.06.001.

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复合万古霉素后对载有BMP2 的仿生磷酸钙骨替代材料成骨性能影响的研究

The influence of vancomycin on osteogenic efficacy of BMP2-coprecipated biomimetic calcium phosphate granules

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