Abstract: Objective: To investigate the effect of hydrophilicity of titanium with micro/nanotopographical sur-face on the cell viability and osteogenic differentiation in human periodontal ligament cells (PDLCs). Methods: Methods of anodic oxidation and sandblast-alkali heat were utilized to establish scale struc-tures. PDLCs were seeded on these two titanium discs, cell viability was detected at 1, 4, 7 and 14 days by CCK8 assay. Total protein values and alkaline phosphatase(ALP) activity were examined at 7 and 14 days. ALP, collagen-I(COL1) and runt related transcription factor 2(RUNX2) mRNA expression at 7 days were detected by qRT-PCR. The date was statistically analyzed with SPSS13.0 software package. Results: Compared with anodic oxidation surfaces, PDLCs on the sandblast-alkali heat surface discs with well hy-drophilicity exhibited enhanced cell viability, increased total protein values, up-regulated ALP activity and overexpression of ALP, COL1 and RUNX2 levels. Conclusion: Micro-nanotopographical surface of titanium implant in sandblast-alkali heat group promotes cell viability and osteogenic differentiation of PDLCs, which may be affected by its hydrophilicity.

Key words: human periodontal ligament cells, osteogenic differentiation, micro-nanotopographical surface, hydrophilicity