Effect of pamidronate disodium on osteogenic differentiation of jaw bone marrow mesenchymal stem cells

doi:10.12337/zgkqzzxzz.2021.08.003

Chinese Journal of Oral Implantology ›› 2021, Vol. 26 ›› Issue (4): 219-225.DOI: 10.12337/zgkqzzxzz.2021.08.003

• Original Articles·Basic Research • Previous Articles Next Articles

Effect of pamidronate disodium on osteogenic differentiation of jaw bone marrow mesenchymal stem cells

Xu Yan^1,2, Sun Jin³, Zhou Haihua⁴

¹Department of Stomatology, Shenzhen Luohu Hospital Group, Guangdong 518000, China;
²The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and the Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Hubei 430079, China;
³Stomatology Health Care Center, Affiliated Shenzhen Maternity, Child Healthcare Hospital, Southern Medical University, Shenzhen University, Guangdong 518000, China;
⁴Department of Oral and Maxillofacial Surgery, College and Hospital of Stomatology, Wuhan University, Hubei 430079, China

Received:2021-06-25 Online:2021-08-10 Published:2021-09-08
Contact: Xu Yan, Email: 635424216@qq.com, Tel: 0086-755-82200110
Supported by:
Shenzhen Luohu District Soft Science Research Plan (LX20201205)

Abstract

Abstract: Objective To explore the cause of bisphosphate-related osteonecrosis by studying the effect of pamidronate disodium (PD) on osteogenic differentiation in jaw bone marrow mesenchymal stem cells (JBMMSCs). Methods JBMMSCs were isolated from the human jaw bone. The concentrations of PD suitable for the growth of JBMMSCs and subsequent studies were screened by thiazolyl blue (MTT) assay at concentrations of 0, 0.1, 0.5, 1.0, 5.0 and 10 μg/ml; The quantification of alizarin red S (ARS) staining and alkaline phosphatase (ALP) staining, and quantitative polymerase chain reaction (qRT-PCR) were used to compare the osteogenic ability of JBMMSCs at different concentrations of PD. Results MTT assay showed that 0.1 and 0.5 μg/ml PD did not affect the growth of JBMMSCs (P>0.05), while 1.0, 5.0 and 10 μg/ mL PD significantly inhibited the growth of JBMMSCs (P<0.05). The quantification of ARS staining and ALP staining, and qPCR showed that the osteogenic ability of JBMMSCs at 0.5 and 0.75 μg/ml of PD was significantly lower than that in the control group (P<0.05). ARS staining and qPCR showed similar osteogenic ability of JBMMSCs treated by 0, 0.1, 0.2, 0.3 or 0.4 μg/ml PD (P>0.05). Conclusion PD may induce osteonecrosis by inhibiting the osteogenic differentiation of JBMMSCs when over a threshold of dose.

Key words: Pamidronate disodium, Mesenchymal stem cells, Osteonecrosis, Osteogenic differentiation

Xu Yan, Sun Jin, Zhou Haihua. Effect of pamidronate disodium on osteogenic differentiation of jaw bone marrow mesenchymal stem cells[J]. Chinese Journal of Oral Implantology, 2021, 26(4): 219-225.

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Effect of pamidronate disodium on osteogenic differentiation of jaw bone marrow mesenchymal stem cells

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