乳酸链球菌素特性及在种植体周炎中应用的研究进展

doi:10.12337/zgkqzzxzz.2025.02.015

摘要/Abstract

摘要： 种植体周炎是种植修复失败的主要原因之一。乳酸链球菌素（nisin）是乳酸菌产生的细菌素,能够通过在细菌胞膜上形成孔道产生抑菌作用,对种植体周炎致病菌有明显抑制作用,具有治疗种植体周炎的潜力。文章就nisin的特性及在种植体周炎应用的研究进展作一综述。

关键词: 乳酸链球菌素, 种植体周炎, 耐药性

Abstract: Peri-implantitis is one of the main causes of implants failure. Nisin is a bacteriocin produced by Lactobacillus, which exerts bacteriostatic effects by forming pores in bacterial cell membranes. Nisin has been shown to effectively inhibit the pathogenic bacteria associated with peri-implantitis, demonstrating its potential for use in the treatment of this condition. This review article discusses the characteristics of nisin and the research progress on its application in peri-implantitis.

Key words: Nisin, Peri-implantitis, Drug resistance

王喻. 乳酸链球菌素特性及在种植体周炎中应用的研究进展[J]. 中国口腔种植学杂志, 2025, 30(1): 86-90. DOI: 10.12337/zgkqzzxzz.2025.02.015

Wang Yu. The characteristics of nisin and its application in peri-implantitis: a review of recent research[J].Chinese Journal of Oral Implantology, 2025, 30(1): 86-90.DOI: 10.12337/zgkqzzxzz.2025.02.015.

参考文献

[1] 文言,王宇蓝.菌斑生物膜与种植体周围炎相关研究进展[J].口腔疾病防治, 2024, 32(9):730-736.DOI:10.12016/j.issn.2096-1456.202330512.
Wen Y, Wang YL .Research progress on the correlation between plaque biofilm and peri-implantitis[J].J Prev Treat Stom Dis,2024,32(9):730-736. DOI: 10.12016/j.issn.2096-1456.202330512.
[2] Field D, Fernandez de Ullivarri M, Ross RP, et al. After a century of nisin research - where are we now?[J]. FEMS Microbiol Rev, 2023,47(3):fuad023.DOI: 10.1093/femsre/fuad023.
[3] Delves-Broughton J, Blackburn P, Evans RJ, et al.Applications of the bacteriocin, nisin[J]. Antonie Van Leeuwenhoek, 1996,69(2):193-202. DOI: 10.1007/BF00399424.
[4] Ibarra-Sánchez LA, El-Haddad N, Mahmoud D, et al.Invited review: advances in nisin use for preservation of dairy products[J]. J Dairy Sci, 2020,103(3):2041-2052. DOI: 10.3168/jds.2019-17498.
[5] Khan F, Singh P, Joshi AS, et al.Multiple potential strategies for the application of nisin and derivatives[J]. Crit Rev Microbiol, 2023,49(5):628-657. DOI: 10.1080/1040841X.2022.2112650.
[6] Wu J, Zang M, Wang S, et al.Nisin: from a structural and meat preservation perspective[J]. Food Microbiol, 2023,111:104207. DOI: 10.1016/j.fm.2022.104207.
[7] Musiejuk M, Kafarski P.Engineering of nisin as a means for improvement of its pharmacological properties: a review[J]. Pharmaceuticals (Basel), 2023,16(8):1058.DOI: 10.3390/ph16081058.
[8] Pérez-Ibarreche M, Field D, Ross RP, et al.A bioengineered nisin derivative to control Streptococcus uberis biofilms[J]. Appl Environ Microbiol, 2021,87(16):e0039121. DOI: 10.1128/AEM.00391-21.
[9] Galván Márquez IJ, McKay B, Wong A, et al. Mode of action of nisin on escherichia coli[J]. Can J Microbiol, 2020,66(2):161-168. DOI: 10.1139/cjm-2019-0315.
[10] Saraiva M, Birri DJ, Brede DA, et al.Nisin Z production by wild strains of Lactococcus iactis isolated from Brazilian (Italian type) fermented sausage[J]. Int J Microbiol, 2020,2020:9309628. DOI: 10.1155/2020/9309628.
[11] Çelen Teyfik,Anumudu Christian,Miri Taghi, et al.Nisin:carboxymethylcellulose polyion complex (PIC) nanoparticles. Preparation and antimicrobial activity[J].Carbohydrate Polymers,2023,317121032-121032.
[12] Roupie C, Labat B, Morin-Grognet S, et al.Nisin-based antibacterial and antiadhesive layer-by-layer coatings[J]. Colloids Surf B Biointerfaces, 2021,208:112121. DOI: 10.1016/j.colsurfb.2021.112121.
[13] Zheng Y, Du Y, Qiu Z, et al.Nisin variants generated by protein engineering and their properties[J]. Bioengineering (Basel), 2022,9(6):251.DOI: 10.3390/bioengineering9060251.
[14] 程琳丽. 乳酸链球菌素的研究现状及在食品中的应用[J].食品安全质量检测学报,2020,11(11):3581-3585.
Cheng LL.Research status and application of nisin in food[J]. J Food Safety Quality,2020,11(11):3581-3585.
[15] Charest AM, Reed E, Bozorgzadeh S, et al.Nisin inhibition of gram-negative bacteria[J]. microorganisms, 2024,12(6):1230.DOI: 10.3390/microorganisms12061230.
[16] 余雪健. N端修饰对Nisin抗菌的影响及在抗菌膜中的应用[D].江苏无锡:江南大学, 2020.DOI:10.27169/d.cnki.gwqgu.2020.001001.
Yu XJ.Effect of N-terminal modification on activity of nisin and its application in antimicrobial film[D].Wuxi:Jiangnan University,2020.DOI:10.27169/d.cnki.gwqgu.2020.001001.
[17] Pimentel-Filho Nde J, Martins MC, Nogueira GB, et al. Bovicin HC5 and nisin reduce Staphylococcus aureus adhesion to polystyrene and change the hydrophobicity profile and Gibbs free energy of adhesion[J]. Int J Food Microbiol, 2014,190:1-8. DOI: 10.1016/j.ijfoodmicro.2014.08.004.
[18] Okuda K, Zendo T, Sugimoto S, et al.Effects of bacteriocins on methicillin-resistant Staphylococcus aureus biofilm[J]. Antimicrob Agents Chemother, 2013,57(11):5572-5579. DOI: 10.1128/AAC.00888-13.
[19] Johnson IH, Hayday H, Colman G.The effects of nisin on the microbial flora of the dental plaque of monkeys (Macaca fascicularis)[J]. J Appl Bacteriol, 1978,45(1):99-109. DOI: 10.1111/j.1365-2672.1978.tb04203.x.
[20] Howell TH, Fiorellini JP, Blackburn P, et al.The effect of a mouthrinse based on nisin, a bacteriocin, on developing plaque and gingivitis in beagle dogs[J]. J Clin Periodontol, 1993,20(5):335-339. DOI: 10.1111/j.1600-051x.1993.tb00369.x.
[21] Turner SR, Love RM, Lyons KM.An in-vitro investigation of the antibacterial effect of nisin in root canals and canal wall radicular dentine[J]. Int Endod J, 2004,37(10):664-671. DOI: 10.1111/j.1365-2591.2004.00846.x.
[22] Le Lay C, Akerey B, Fliss I, et al.Nisin Z inhibits the growth of Candida albicans and its transition from blastospore to hyphal form[J]. J Appl Microbiol, 2008,105(5):1630-1639. DOI: 10.1111/j.1365-2672.2008.03908.x.
[23] Pinheiro ET, Karygianni L, Attin T, et al.Antibacterial effect of high-purity nisin alone and in combination with d-amino acids or chlorhexidine in an endodontic-like biofilm model[J]. Antibiotics (Basel), 2021,10(2):1490.DOI: 10.3390/antibiotics10020149.
[24] Tong Z, Zhou L, Li J, et al.In vitro evaluation of the antibacterial activities of MTAD in combination with nisin against Enterococcus faecalis[J]. J Endod, 2011,37(8):1116-1120. DOI: 10.1016/j.joen.2011.03.020.
[25] Cunha E, Janela R, Costa M, et al.Nisin influence on the antimicrobial resistance ability of canine oral enterococci[J]. Antibiotics (Basel), 2020,9(12):890.DOI: 10.3390/antibiotics9120890.
[26] Enigk K, Jentsch H, Rodloff AC, et al.Activity of five antimicrobial peptides against periodontal as well as non-periodontal pathogenic strains[J]. J Oral Microbiol, 2020,12(1):1829405. DOI: 10.1080/20002297.2020.1829405.
[27] Wu M, Ma Y, Dou X, et al.A review of potential antibacterial activities of nisin against Listeria monocytogenes: the combined use of nisin shows more advantages than single use[J]. Food Res Int, 2023, 164:112363. DOI: 10.1016/j.foodres.2022.112363.
[28] Shi Y, Wen T, Zhao F, et al.Bacteriostasis of nisin against planktonic and biofilm bacteria: its mechanism and application[J]. J Food Sci, 2024,89(4):1894-1916. DOI: 10.1111/1750-3841.17001.
[29] Guo H, Zhao F, Lei B, et al.Synergistic antimicrobial system based on nisin and α-hydroxy organic acids[J]. Arch Microbiol, 2023,205(6):225. DOI: 10.1007/s00203-023-03572-2.
[30] Bermudez-Aguirre D, Niemira BA.Effect of nisin, EDTA, and abuse temperature on the growth of Salmonella typhimurium in liquid whole egg during refrigerated storage[J]. Food Res Int, 2023,174(Pt 1):113568. DOI: 10.1016/j.foodres.2023.113568.
[31] 吴梦楠. MTAN对种植体周围炎致病菌及生物膜抑制作用的体外研究[D].河南:郑州大学, 2019.
Wu MN.Study of MTAN to inhibit the pathogenic bacteria and biofilm of peri-implantitis in vitro[D]. Henan : Zhengzhou University, 2019.
[32] Kuwano K, Tanaka N, Shimizu T, et al.Dual antibacterial mechanisms of nisin Z against Gram-positive and Gram-negative bacteria[J]. Int J Antimicrob Agents, 2005,26(5):396-402. DOI: 10.1016/j.ijantimicag.2005.08.010.
[33] Shin JM, Ateia I, Paulus JR, et al.Antimicrobial nisin acts against saliva derived multi-species biofilms without cytotoxicity to human oral cells[J]. Front Microbiol, 2015,6:617. DOI: 10.3389/fmicb.2015.00617.
[34] Pag U, Sahl HG.Multiple activities in lantibiotics--models for the design of novel antibiotics?[J]. Curr Pharm Des, 2002,8(9):815-833. DOI: 10.2174/1381612023395439.
[35] Radaic A, Brody H, Contreras F, et al.Nisin and Nisin probiotic disrupt oral pathogenic biofilms and restore their microbiome composition towards healthy control levels in a peri-implantitis setting[J]. Microorganisms, 2022,10(7):1336.DOI: 10.3390/microorganisms10071336.
[36] Radaic A, Ye C, Parks B, et al.Modulation of pathogenic oral biofilms towards health with nisin probiotic[J]. J Oral Microbiol, 2020,12(1):1809302. DOI: 10.1080/20002297.2020.1809302.
[37] Kuraji R, Ye C, Zhao C, et al.Nisin lantibiotic prevents NAFLD liver steatosis and mitochondrial oxidative stress following periodontal disease by abrogating oral, gut and liver dysbiosis[J]. NPJ Biofilms Microbiomes, 2024,10(1):3. DOI: 10.1038/s41522-024-00476-x.
[38] Gao L, Kuraji R, Zhang MJ, et al.Nisin probiotic prevents inflammatory bone loss while promoting reparative proliferation and a healthy microbiome[J]. NPJ Biofilms Microbiomes, 2022,8(1):45. DOI: 10.1038/s41522-022-00307-x.
[39] Cunha E, Valente S, Nascimento M, et al.Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study[J]. PeerJ, 2021,9:e11626. DOI: 10.7717/peerj.11626.
[40] Birk SE, Mosgaard MD, Kjeldsen RB, et al.Management of oral biofilms by nisin delivery in adhesive microdevices[J]. Eur J Pharm Biopharm, 2021,167:83-88. DOI: 10.1016/j.ejpb.2021.07.007.
[41] Shin JM, Ateia I, Paulus JR, et al.Antimicrobial nisin acts against saliva derived multi-species biofilms without cytotoxicity to human oral cells[J]. Front Microbiol, 2015,6:617. DOI: 10.3389/fmicb.2015.00617.
[42] 蔡文静. 亚甲基蓝介导的光动力疗法与MTAN联合应用于抑制具核梭杆菌以及其生物膜的体外研究[D].河南:郑州大学,2021.
Cai WJ.The combined application of methylene blue-mediated photodynamic therapy and MTAN to inhibition Fusobacterium nucleoli and its biofilm in vitro[D]. Henan:Zhenzhou University ,2021.
[43] Mitra D, Yadav A, Prithyani S, et al.The antiplaque efficacy of lantibiotic nisin extract mouthrinse[J]. J Indian Soc Periodontol, 2019,23(1):31-34. DOI: 10.4103/jisp.jisp_326_18.
[44] 伟日(山东)生物科技有限公司. 有效抑菌的漱口水及制备方法:中国,CN201910968045.0[P].2019-12-03.
Weiri (Shandong) Biotechnology Co., Ltd.Effective antibacterial mouthwash and preparation method: China,CN201910968045.0[P].2019-12-03.
[45] 山东省药学科学院. 一种含有乳酸链球菌素、聚赖氨酸的口腔抑菌组合物及其制备方法:中国,CN201510714037.5[P].2016-02-17.
Shandong Academy of Pharmaceutical Sciences.An oral bacteriostatic composition containing lactistreptococcin polylysine and a preparation method thereof: China,CN201510714037.5[P].2016-02-17.