既往种植牙合并复发性口腔溃疡患者的CD3+、CD4+、CD8+、IL-2、IL-6、TNF-α 水平变化分析研究

doi:10.12337/zgkqzzxzz.2019.06.002

摘要/Abstract

摘要： 目的： 研究既往种植牙合并复发性口腔溃疡患者CD3+、CD4+、CD8+、IL-2、IL-6、TNF-α水平的变化情况。方法： 将从2016年2月至2018年12月我院收治的既往种植牙合并复发性口腔溃疡患者60例作为观察对象,记为试验组。另取同期于我院进行健康体检的既往种植牙人员60例记为对照组。分别采用流式细胞仪检测两组人员CD3+、CD4+、CD8+水平,并计算CD4+/CD8+值,同时,采用酶联免疫吸附法检测两组人员血清白细胞介素-2(IL-2)、白细胞介素-6(IL-6)以及肿瘤坏死因子-α(TNF-α)水平。并作相关性分析。结果： 试验组患者CD4+、CD8+水平分别为(35.01± 4.23)%、(33.22± 5.37)%,相比对照组的(31.76± 3.82)%、(22.17± 3.98)%较高,而CD4+/CD8+水平为(1.26± 0.61),相比对照组的(1.87± 0.89) 较低(均P< 0.05)。试验组患者血清IL-2、IL-6、TNF-α水平分别为(39.77± 3.58)pg/ml、(2.81± 0.50)pg/ml、(15.59± 3.79)pg/ml,相比对照组的(23.05± 10.24)pg/ml、(0.72± 0.15)pg/ml、(10.24± 1.28)pg/ml较高(均P< 0.05)。经Pearson相关性分析可得：既往种植牙合并复发性口腔溃疡患者CD4+、CD8+水平与血清IL-2、IL-6、TNF-α水平均呈正相关,而CD4+/CD8+水平与血清IL-2、IL-6、TNF-α水平均负相关(均P< 0.05)。结论： 既往种植牙合并复发性口腔溃疡患者CD4+、CD8+水平呈明显高表达,而CD4+/CD8+水平呈明显低表达,提示了免疫功能紊乱可能与复发性口腔溃疡的发生、发展存在密切相关。临床工作中可能通过联合检测血清IL-2、IL-6、TNF-α水平以反映患者的免疫功能。;

关键词: 复发性口腔溃疡, 既往种植牙, 白细胞介素, 肿瘤坏死因子-α, 免疫功能

Abstract: Objective： To study and analyze the changes of CD3、CD4+、CD8+、IL-2、IL-6 and TNF-α levels in patients with recurrent oral ulcer treated with dental implants. Methods： From February 2016 to Decem- ber 2018, 60 patients with previous dental implants and recurrent oral ulcer admitted to our hospital as ob- servation objects were recorded as the experimental group. In addition, 60 cases of previous dental im- plant personnel who underwent physical examination in our hospital in the same period were recorded as the control group. CD3+, CD4+, and CD8+ levels were detected by flow cytometry, and CD4+/CD8+ values were calculated. Meanwhile, serum levels of interleukin-2(IL-2), interleukin-6 (IL-6) and tumor necro- sis factor-α(TNF-α) were detected by enzyme-linked immunosorbent assay. Correlation analysis was al- so maken. Results： The CD4+ and CD8+ levels in the experimental group were(35.01±4.23)% and(33.22± 5.37) %, respectively higher than the control group(31.76±3.82)% and(22.17±3.98)%. The CD4+/CD8+ level(1.26±0.61) was lower than the control group (1.87±0.89) (all P<0.05). The serum IL-2, IL-6 and TNF-α levels in the experimental group were(39.77±3.58) pg/ml, (2.81±0.50) pg/ml, (15.59±3.79) pg/ml, compared with the control group(23.05±10.24) pg/ml, (0.72±0.15) pg/ml, (10.24±1.28) pg/ml was higher (all P<0.05). Pearson correlation analysis showed that CD4+ and CD8+ levels were positively correlated with serum IL-2, IL-6 and TNF-α levels, while CD4+/CD8+ levels were negatively correlated with serum IL-2, IL-6 and TNF-α levels(all P<0.05). Conclusion： CD4+ and CD8+ levels were significantly higher in patients with dental implants and recurrent oral ulcer, while CD4+/CD8+ levels were significantly lower, suggesting that immune dysfunction may be closely related to the occurrence and development of recur- rent oral ulcer. In clinical work, serum IL-2, IL-6 and TNF-α levels may be jointly detected to reflect the immune function of patients.

Key words: recurrent oral ulcer, previous dental implants, interleukines, tumor necrosis factor-α, immune function

中图分类号:

781.5

师海瑞,孔义华,王显河,等. 既往种植牙合并复发性口腔溃疡患者的CD3+、CD4+、CD8+、IL-2、IL-6、TNF-α 水平变化分析研究[J]. 中国口腔种植学杂志, 2019, 24(2): 55-58. DOI: 10.12337/zgkqzzxzz.2019.06.002

SHI Hairui, KONG Yihua, WNAG Xianhe, et al. Analysis on the changes of CD3+、CD4+、CD8+、IL-2、IL-6 and TNF-α levels in patients with recurrent oral ulcer treated with dental implants[J].Chinese Journal of Oral Implantology, 2019, 24(2): 55-58.DOI: 10.12337/zgkqzzxzz.2019.06.002.

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