珊瑚羟基磷灰石圆柱体骨传导能力的初步动物研究

doi:10.12337/zgkqzzxzz.2018.06.002

摘要/Abstract

摘要： 目的： 通过将可降解珊瑚羟基磷灰石圆柱体植入大白兔股骨远中极限骨缺损中,探讨珊瑚羟基磷灰石圆柱体新骨的形成情况,为种植体植入的时机提供参考。方法： 将30只新西兰大白兔分为实验组24只和对照组6只。实验组,在兔子股骨远中干骺端植入48块珊瑚羟基磷灰石,在4周、8周、12周时分别处死8只,行大体标本观察和组织学分析; 对照组,饲养至12周时全部处死,行相应分析。结果： 实验组24只大白兔均愈合良好,4周时仅植入物边缘有少量新骨形成,8周时植入物中心有明显新生骨长入,12周时植入物边缘与中心新骨形成均增加。实验组新生骨面积百分比,4周与8周之间差异具有统计学意义,8周与12周时无明显差别。对照组缺损中心仅有少量炎症细胞和成纤维细胞长入,无骨组织形成。结论： 可降解珊瑚羟基磷灰石圆柱体植入兔子股骨远中极限骨缺损中后愈合良好,8周时骨块中心有新骨显著形成,此时,适合种植体的植入。

关键词: 珊瑚羟基磷灰石圆柱体, 极限骨缺损, 新骨面积百分比, 骨传导性

Abstract: Objective: The biodegradable coralline hydroxyapatite cylinder was implanted into the bone defect of the distal femur of rabbits, and the formation of new bone of coralline hydroxyapatite cylinder was dis-cussed, so as to provide references for the timing of implant placement. Methods: 30 white New Zealand rabbits were randomly divided into 24 rabbits in the test group and 6 in the control group. In the experi-mental group, 48 coralline hydroxyapatite were implanted in the distal femur of the rabbit, and 8 rabbits were sacrificed at 4, 8 and 12 weeks, respectively. Gross specimens were observed and histologically ana-lyzed. In the control group, all the animals were killed at 12 weeks, and the corresponding analysis was performed. Results: The 24 rabbits in the test group healed well. At the 4th week, there was only a few new bone formation at the edge of the implant. At the 8th week, there was obvious new bone ingrowth in the implant center. At the 12th week, both the edge of the implant and the new bone in the center in-creased. The percentage of new bone area in the test group was statistically significant between the 4th week and the 8 week, and there was no significant difference between the 8 week and the 12 week. In the control group, only a small amount of inflammatory cells and fibroblasts grew into the defect center, and no bone tissue was formed. Conclusion: The biodegradable coralline hydroxyapatite cylinder healed well after implantation into the bone defect of rabbit femur, and at 8 weeks, new bone was formed in the center of the bone, which is suitable for implant placement.

Key words: coralline hydroxyapatite cylinder, critical bone defect, percentage of new bone area, os-teoconduction

中图分类号:

782.13

姚钟雄,等徐淑兰,等邵军. 珊瑚羟基磷灰石圆柱体骨传导能力的初步动物研究[J]. 中国口腔种植学杂志, 2018, 23(2): 57-60. DOI: 10.12337/zgkqzzxzz.2018.06.002

YAO Zhongxiong, XU Shulan, SHAO Jun. A preliminary animal study on osteoconduction capacity of coralline hydroxyapatite cylinders[J].Chinese Journal of Oral Implantology, 2018, 23(2): 57-60.DOI: 10.12337/zgkqzzxzz.2018.06.002.

参考文献

[1] Vercellotti T,De Paoli S,Nevins M.The piezoelec-tric bony window osteotomy and sinus membrane elevation:in-troduction of a new technique for simplification of the sinus aug-mentation procedure[J]. Int J Periodontics Restorative Dent, 2001, 21(6):561-567
[2] Rocchietta I, Fontana F, Simion M.Clinical out-comes of vertical bone augmentation to enable dental implant placement: a systematic review[J]. J Clin Periodontol, 2008, 35(8): 203-215
[3] 杨烁,徐淑兰,姚钟雄,等. 可降解珊瑚羟基磷灰石与脱矿无机牛骨在上颌窦提升术后改建的临床对比研究[J]. 实用口腔医学杂志, 2013, 29(3): 334-338
[4] Michele F, Francesco G, Leonzio F, et al.Vertical Ridge Augmentation of the Atrophic Posterior Mandible with Custom-Made, Computer-Aided Design/Computer-Aided Manu-facturing Porous Hydroxyapatite Scaffolds[J]. J Craniofac Surg, 2013, 24(3): 856-860
[5] 姚钟雄,徐淑兰,王朝,等. 块状珊瑚羟基磷灰石重建下颌骨高度缺损的临床效果[J]. 广东医学, 2014, 35(8): 1229-1232
[6] Ono D, Jimbo R, Kawachi G, et al.Lateral bone aug-mentation with newly developed beta-tricalcium phosphate block: an experimental study in the rabbit mandible[J]. Clin Oral Implants Res, 2011, 22(12): 1366-1371
[7] 韦从云, 周磊, 赖春花, 等. 骨代用品实验中兔股骨远端标准骨缺损模型的建立[J]. 广东牙病防治, 2010, 18(6): 304-308
[8] Torres J, Tamimi F, Alkhraisat MH, et al.Vertical bone augmentation with 3D-synthetic monetite blocks in the rab-bit calvaria[J]. J Clin Periodontol, 2011, 38(12): 1147-1153
[9] 高文峰,许竞,钟永荣. 羟基磷灰石生物陶瓷在上颌埋伏尖牙拔除术中的应用[J]. 广东牙病防治, 2012, 20(5): 261-263
[10] 李娟, 郑闱颖, 卢岩, 等. 骨形成蛋白-2基因修饰的β磷酸三钙/胶原复合材料修复颅骨缺损[J]. 材料科学与工程学报, 2013, 12(02): 248-252
[11] 陈钢, 李树春, 马练, 等. 下颌骨取骨onlay植骨改善种植骨量不足的临床研究[J]. 口腔颌面外科杂志, 2005, 15(2): 166-169
[12] Ning Y, Wei T, Defu C, et al.The research of degrad-ability of a novel biodegradable coralline hydroxyapatite after im-planted into rabbit[J]. J Biomed Mater Res A, 2009, 88(3): 741-746
[13] Kubo T, Doi K, Hayashi K, et al. Comparative evalua-tion of bone regeneration using spherical and irregularly shaped granules of interconnected porous hydroxylapatite[J]. J Prostho-dont Res, 2011, 55(2): 104-109
[14] Naaman BAN, Patat JL, Guillemin G, et al.Evalua-tion of the osteogenic potential of biomaterials implanted in the palatal connectivetissue of miniaturepigs using undecalcified sections[J]. Biomaterials, 1994, 2(15): 201-202
[15] Tania MC,Jose MG,Gerson FA,et al.Bone repair and augmentation using block of sintered bovine-derived anorganic bone graft in cranial bone defect model[J]. Clin Oral Impl Res. 20, 2009, 20: 340-350
[16] 宋亚平,徐世同,刘虎,等. 4种骨移植材料修复骨缺损动物模型的建立[J]. 广东牙病防治, 2014, 22(5): 229-233