新型富胶原蛋白骨基质在位点保存术中的应用效果研究

doi:10.12337/zgkqzzxzz.2019.09.006

摘要/Abstract

摘要： 目的： 评价小牛脱细胞松质骨基质在位点保存术中的成骨效果。方法： 选择纯种雄性Beagle犬16只,随机分成四组,每组4只。分别拔除每只Beagle犬的下颌第三、第四前磨牙,各组拔牙窝分别放置小牛脱细胞松质骨基质、Bio-oss骨粉、Bio-oss骨粉+CGF充填及空白对照,胶原膜覆盖后,严密缝合。术后四组分别于8周和12周时处死Beagle犬,通过CBCT观察牙槽骨高度及宽度的变化,通过ABA专用骨骼分析软件显微CT检测测定比较各组组织骨密度,骨小梁数目及骨材料吸收速率。通过HE及Masson染色观察并比较各组间新骨形成、骨改建及骨粉吸收情况,同时观察不同时期血管形成、类骨质矿化、骨小梁形成及炎症反应状况。结果： BABM、Biooss骨粉、Bio-oss+CGF术前术后牙槽骨高度比较差异无统计学意义(Z=-1.475,P=0.140),术后8周、12周均高于空白对照组BABM、Bio-oss骨粉、Bio-oss+CGF和空白组术前术后牙槽骨宽度比较,术后8周、12周较术前及术后当日低,差异具有统计学意义(Z=-3.716,P< 0.05);拔牙后植入小牛脱细胞松质骨基质组、Bio-oss 组和Biooss+CGF组12周时骨高度和宽度吸收率无统计学差异,组织形态学显示Bio-oss+CGF组与小牛脱细胞松质骨基质组8周时新骨形成率高于Bio-oss 组。结论： 小牛脱细胞松质骨基质在拔牙窝保存术中能有效维持牙槽骨的高度和宽度,同时能促进牙槽骨的早期生成。

关键词: CGF, Bio-oss 骨粉, 小牛脱细胞松质骨基质, 位点保存

Abstract: Objective： To evaluate the osteogenic effect of acellular cancellous bone matrix in site preservation of calf. Methods： We select sixteen pure male Beagle dogs, and then randomly divide them into four groups, four in each group. The third and fourth premolars of each Beagle dog were extracted. Then place Calf acellular cancellous bone matrix, Bio-oss bone, Bio-oss bone powder+CGF and blank control in the extraction socket of each group. After the teeth extraction sockets were covered with collagen membrane, and sutured tightly. Beagle dogs were sacrificed at 8 and 12 weeks after operation. The height and width of alveolar bone were observed by CBCT. Bone mineral density (BMD), number of trabeculae and absorption rate of bone materials were measured by ABA bone analysis software. New bone formation, bone remodeling and bone powder absorption were observed and compared by HE and Masson staining, meanwhile the Vascularization, osteoid mineralization, trabecular bone formation and inflammatory reaction were also observed at different stages. Results： There was no statistical significance in alveolar bone height between BABM, Bio-oss bone powder and Bio-oss+CGF tion.(Z=-1.475, P=0.140). The levels of BABM, Bio-oss bone powder, Bio-oss+CGF and blank group were higher at 8 and 12 weeks after operation than those in blank control group. The alveolar bone width before and after operation was lower than that before and on the day after operation at 8 and 12 weeks after operation. The difference has statistical significance(Z=-3.716,P<0.05). There was no statistical difference in bone height and width absorptivity between the acellular cancellous bone matrix group, the Bio-oss group and the Bio-oss+CGF group at 12 weeks after tooth extraction. Histomorphology showed that the new bone formation rate in the Bio-oss+CGF group and the acellular cancellous bone matrix group at 8 weeks was higher than that in the Bio-oss group. Conciusion: Calf acellular cancellous bone matrix can effectively maintain the height and width of alveolar bone and promote the early formation of alveolar bone during the preservation of extraction socket.

Key words: CGF, Bio-oss bone meal, calf acellular cancellous bone matrix, site preservation

中图分类号:

783.4

朱洪光,白建文,李倜,等. 新型富胶原蛋白骨基质在位点保存术中的应用效果研究[J]. 中国口腔种植学杂志, 2019, 24(3): 123-126. DOI: 10.12337/zgkqzzxzz.2019.09.006

ZHU Hongguang, BAI Jianwen, LI Ti, et al. Study on the application effect of new collagen-rich bone matrix in site preservation[J].Chinese Journal of Oral Implantology, 2019, 24(3): 123-126.DOI: 10.12337/zgkqzzxzz.2019.09.006.

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