浓缩生长因子(CGF)与Bio-oss 骨粉混合应用于位点保存的效果研究

doi:10.12337/zgkqzzxzz.2020.12.001

摘要/Abstract

摘要： 目的: 将浓缩生长因子(CGF)与Bio-oss以不同比例混合,探讨何种比例可以达到更佳的位点保存效果。方法: 24只雄性新西兰兔下颌双侧第一前臼齿拔牙窝,左侧于右侧手术8周后进行。A、B、C组为CGF与Bio-oss以2：1,1：1,1：2比例混合,D组为空白对照。观察4周、12周新骨形成比例及骨成熟程度,X线片计算牙槽骨吸收量。结果: 同一时间点,新骨形成量A>B>C>D（P<0.05）,A、B、C 组牙槽骨吸收量均小于D 组（P<0.05）。同一组别,12周时新生骨量、骨成熟程度及牙槽骨吸收量均高于4周时（P<0.05）。结论: CGF与Bio-oss以2：1比例混合时位点保存效果更佳。

关键词: 浓缩生长因子, Bio-oss骨粉, 位点保存

Abstract: Objective: Different ratios of CGF and Bio-oss were filled in teeth extraction socket, to explore the ratio of better site preservation effect. Methods: 24 male New Zealand rabbits, the experiment has been approved by the ethics committee of Hospital of Stomatology Hebei Medical University. Groups A, B and C were CGF mixed with Bio-oss at the ratio of 2:1, 1:1 and 1:2. Group D was control group. The teeth extraction socket of the first premolar on both sides of mandible was filled with the same ratio. The left operation was performed on 8 weeks after the right operation, and then they were sacrificed after 4 weeks.Amount of new bone formation and the degree of bone maturity were observed histologically at 4 and 12 weeks using HE and Masson stain. X-rays were used to evaluate the alveolar bone absorption.All images were analyzed by image-pro Plus6.0. Results: At the same time, the order of was group A, B, C and D(P<0.05), alveolar bone resorption in group A, B and C was smaller than group D(P<0.05). At the same group, the new bone formation, bone mass, alveolar bone resorption at 12 weeks was higher than that at 4 week（P<0.05）. Conclusion: When CGF and Bio-oss were mixed in the ratio of 2:1 was better.

Key words: concentrated growth factor(CGF), Bio-oss, site preservation

中图分类号:

R782.1

李贝贝,陈志宇,袁硕,等. 浓缩生长因子(CGF)与Bio-oss 骨粉混合应用于位点保存的效果研究[J]. 中国口腔种植学杂志, 2020, 25(4): 151-154. DOI: 10.12337/zgkqzzxzz.2020.12.001

LI Beibei, CHEN Zhiyu, YUAN Shuo, et al. Effect of CGF and Bio-oss ratio on site preservation[J].Chinese Journal of Oral Implantology, 2020, 25(4): 151-154.DOI: 10.12337/zgkqzzxzz.2020.12.001.

参考文献

1 Schar M O, Diaz-Romero J, Kohl S, et al. Plateletrich Concentrates Differentially Release Growth Factors and Induce Cell Migration In Vitro[J]. Clinical Orthopaedics and Related Research , 2015, 473(5): 1635-1643
2 Lundquist R, Dziegiel M H, Agren M S. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin[J]. Wound Repair Regen, 2008, 16(3): 356-363
3 Dohan Ehrenfest D M, de Peppo G M, Doglioli P, et al. Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin(PRF): a gold standard to achieve for all surgical platelet concentrates technologies[J]. Growth Factors, 2009, 27(1): 63-69
4 Bolukbasi N, Ersanli S, Keklikoglu N, et al. Sinus Augmentation With Platelet-Rich Fibrin in Combination With Bovine Bone Graft Versus Bovine Bone Graft in Combination With Collagen Membrane[J]. J Oral Implantol, 2015, 41(5): 586-595
5 Ocak, H, Kutuk, N, Demetoglu, U, Balcloglu, E, Ozdamar,S, & Alkan, A.(2017). Comparison of Bovine Bone-Autogenic Bone Mixture Versus Platelet-Rich Fibrin for Maxillary Sinus Grafting: Histologic and Histomorphologic Study. Journal of Oral Implantology, 43(3):194-201
6 Hatano N, Shimizu Y, Ooya K. A clinical long-term radiographic evaluation of graft height changes after maxillary sinus floor augmentation with a 2 : 1 autogenous bone/xenograft mixture and simultaneous placement of dental implants[J]. Clinical Oral Implants Research, 2004, 15(3): 339-345
7 Dohan Ehrenfest D M, Diss A, Odin G, et al. In vitro effects of Choukroun's PRF(platelet-rich fibrin)on human gingival fibroblasts, dermal prekeratinocytes, preadipocytes, and maxillofacial osteoblasts in primary cultures[J]. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2009, 108(3): 341-352
8 Dohan Ehrenfest D M, Doglioli P, de Peppo G M, et al. Choukroun's platelet-rich fibrin(PRF)stimulates in vitro proliferation and differentiation of human oral bone mesenchymal stem cell in a dose-dependent way[J]. Archives of Oral Biology, 2010, 55(3): 185-194
9 Chang I C, Tsai C H, Chang Y C. Platelet-rich fibrin modulates the expression of extracellular signal-regulated protein kinase and osteoprotegerin in human osteoblasts[J]. Journal of Biomedical Materials Research Part A, 2010,95A(1): 327-332
10 Suba Z. Alveolar Bone Regeneration by Plate-Pich Plasma in Beagle Dogs: A Histologic and Histomorphometric Study[J]. Oral Maxillofac Implants, 2004
11 Bolukbasi N, Ersanli S, Keklikoglu N, et al. Sinus Augmentation With Platelet-Rich Fibrin in Combination With Bovine Bone Graft Versus Bovine Bone Graft in Combination With Collagen Membrane[J]. J Oral Implantol, 2015, 41(5): 586-595
12 Nguyen T, Palankar R, Bui V, et al. Rupture Forces among Human Blood Platelets at different Degrees of Activation[J]. Scientific Reports, 2016, 6(1)
13 Duda M, Paj ak J. The issue of bioresorption of the Bio-Oss xenogeneic bone substitutein bone defects[J]. Ann Univ Mariae Curie Sklodowska[Med], 2004, 59(1): 269-277

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