Abstract: Objective: To investigate the effect of p53/CXCL12 on the osteogenic differentiation of periodontal ligament cells(PDLCs) induced by hydrophilicity of titanium with micro/nanotopographical surface. Methods: Anodic oxidation and sandblast-alkali heat methods were used to establish scale structures, which were cultured with PDLCs. Expressions of p53 and CXCL12 were examined by western blot at 7 day. After transfection of p53 or CXCL12 with siRNA, p53 and CXCL12 level were furthered detected, changes of alkaline phosphatase(ALP) activity and mRNA expressions of such osteogenic markers as ALP, collagen- I(COL1) and runt related transcription factor 2(RUNX2) were examined to detect osteogenic differentiation of PDLCs. Date was statistically analyzed with SPSS13.0 software package. Results: Compared with anodic oxidation surfaces, p53 expression of PDLCs on sandblast-alkali heat surface discs with well hydrophilicity was significantly decreased, while CXCL12 level increased. Transfected with Si-p53, p53 level was decreased and CXCL12 level increased. After transfection of Si-CXCL12, CXCL12 level was decreased, but no significant change of p53 level. However, ALP activity and ALP, COL1 and RUNX2 mRNA levels were significantly decreased. Conclusion: Hydrophilicity of titanium with micro/nanotopographical surface promotes osteogenic differentiation of PDLCs via p53/CXCL12.

Key words: hydrophilicity, periodontal ligament cells, p53, CXCL12, osteogenic differentiation, micro- nanotopographical surface