3D打印镍钛植入物表面去合金化处理及细胞相容性研究

doi:10.12337/zgkqzzxzz.2023.04.002

中国口腔种植学杂志 ›› 2023, Vol. 28 ›› Issue (2): 77-81.DOI: 10.12337/zgkqzzxzz.2023.04.002

3D打印镍钛植入物表面去合金化处理及细胞相容性研究

秦欣玉¹, 张亮¹, 陈莹莹¹, 韩泽奎¹, 宿玉成^1,2,3, 王心彧¹

¹黑龙江省口腔生物材料与临床应用重点实验室佳木斯大学口腔医学工程实验中心佳木斯大学附属口腔医学院 154002;
²北京瑞城口腔医院北京口腔种植培训中心 100032;
³中国医学科学院北京协和医院口腔种植中心 100032

收稿日期:2023-01-06 出版日期:2023-04-30 发布日期:2023-05-04
通讯作者: 王心彧,Email：wxysgs123@126.com,电话：0454-8627689
作者简介:秦欣玉,硕士研究生在读,研究方向：金属3D打印与表面处理;王心彧,副主任医师、硕士研究生导师,研究方向：数字化口腔种植、金属3D打印与表面处理

Study on surface dealloying treatment and cytocompatibility of 3D printed nickel-titanium implants

Qin Xinyu¹, Zhang Liang¹, Chen Yingying¹, Han Zekui¹, Su Yucheng^1,2,3, Wang Xinyu¹

¹Key Laboratory of Oral Biomaterials and Clinical Applications of Heilongjiang Province, Stomatology Engineering Experimental Center of Jiamusi University, School of Stomatology, Jiamusi University 154002, China;
²Beijing Citident Hospital of Stomatology, Beijing Implant Training College (BITC), Beijing 100032, China;
³Dental Implant Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100032, China

Received:2023-01-06 Online:2023-04-30 Published:2023-05-04
Contact: Wang Xinyu, Email:wxysgs123@126.com, Tel:0086-454-8627689

摘要/Abstract

摘要： 目的本文通过喷砂、酸蚀、去合金等生物活性处理方法研究3D打印镍钛合金的最佳改性工艺。方法利用扫描电镜、能谱仪和接触角测量等对试件的表面结构、性能进行检测;本实验用人骨髓间充质干细胞（human bone marrow mesenchymal stem cells,hBMSCs）对不同表面的生物相容性及成骨活性进行研究。结果去合金化表面亲水性得到了提高,能谱仪分析结果显示表面镍元素从最初的52.1 wt%降至18.6 wt%,不但能加速细胞在其表面的分化和增殖,而且能增强细胞的附着能力。结论去合金表面构建了更加丰富的纳米级网架结构,对细胞成骨分化和功能的表达具有明显促进作用,这对提高骨结合能力和植入体的长期稳定具有重要意义。

关键词: 镍钛合金, 表面处理, 去合金化, 细胞相容性

Abstract: Objective This paper investigates the optimal modification process of 3D printed nickel-titanium alloys by bioactive treatments such as sandblasting, acid etching, and dealloying. Methods The surface structure and properties of specimens were examined by scanning electron microscope (SEM), energy dispersive spectrometer (EDS) and contact angle measurement. In this study, bone marrow mesenchymal stem cells (hBMSCs) were used to study the biocompatibility and osteogenic activity of different surfaces. Results The hydrophilicity of the dealloying surface was improved. EDS results showed that the surface nickel elementdecreased from 52.1wt% to 18.6wt%, which not only accelerated the differentiation and proliferation of cells on the surface, but also enhanced the adhesion ability of cells. Conclusion The dealloyed surface constructed a more abundant nanoscale mesh structure, which significantly promoted the osteogenic differentiation and functional expression of cells, which was of great importance for improving the osseointegration ability and long-term stability of implants.

Key words: Nickel-titanium alloy, Surface treatment, Dealloying, Cytocompatibility

秦欣玉,张亮,陈莹莹,等. 3D打印镍钛植入物表面去合金化处理及细胞相容性研究[J]. 中国口腔种植学杂志, 2023, 28(2): 77-81. DOI: 10.12337/zgkqzzxzz.2023.04.002

Qin Xinyu, Zhang Liang, Chen Yingying, Han Zekui, Su Yucheng, Wang Xinyu. Study on surface dealloying treatment and cytocompatibility of 3D printed nickel-titanium implants[J].Chinese Journal of Oral Implantology, 2023, 28(2): 77-81.DOI: 10.12337/zgkqzzxzz.2023.04.002.

参考文献

[1] Mohd Jani J, Leary M, Subic A, et al.A review of shape memory alloy research, applications and opportunities[J]. Materials and Design, 2014,56:1078-1113.DOI:10.1016/j.matdes.2013.11.084
[2] 谭树松. 形状记忆合金研究的最新进展及应用[J].功能材料,1991,3:185-190,192.
[3] Patel S K, Behera B, Swain B, et al.A review on NiTi alloys for biomedical applications and their biocompatibility[J]. Materials Today: Proceedings, 2020,33:5548-5551.DOI:10.1016/j.matpr.2020.03.538.
[4] Mani G, Porter D, Grove K, et al.Surface finishing of nitinol for implantable medical devices: a review[J]. J Biomed Mater Res B Appl Biomater, 2022,110(12):2763-2778. DOI: 10.1002/jbm.b.35112.
[5] Nair VS, Nachimuthu R.The role of NiTi shape memory alloys in quality of life improvement through medical advancements: a comprehensive review[J]. Proc Inst Mech Eng H, 2022,236(7):923-950. DOI: 10.1177/09544119221093460.
[6] Bhagyaraj J, Ramaiah K V, Saikrishna C N, et al.Behavior and effect of Ti2Ni phase during processing of NiTi shape memory alloy wire from cast ingot[J].Journal of Alloys and Compounds , 2013,581:344-351.
[7] Močnik P, Kosec T.A critical appraisal of the use and properties of nickel-titanium dental alloys[J]. Materials (Basel), 2021,14(24):7859. DOI: 10.3390/ma14247859.
[8] Bogdanski D, Epple M, Esenwein S A, et al.Biocompatibility of calcium phosphate-coated and of geometrically structured nickel-titanium (NiTi) by in vitro testing methods[J]. Materials Science & Engineering. A, Structural Materials : Properties, Microstructure and Processing, 2004,378(1): 527-531. DOI:10.1016/j.msea.2003.11.071
[9] Wu SL, Chu PK, Liu XM, et al.Surface characteristics, mechanical properties, and cytocompatibility of oxygen plasma-implanted porous nickel titanium shape memory alloy[J]. J Biomed Mater Res A, 2006,79(1):139-146. DOI: 10.1002/jbm.a.30705.
[10] Kim J, Park JK, Kim HK, et al.Optimization of electropolishing on NiTi alloy stents and its influence on corrosion behavior[J]. J Nanosci Nanotechnol, 2017,17(4):2333-2339. DOI: 10.1166/jnn.2017.13324.
[11] Mohammadi F, Golafshan N, Kharaziha M, et al.Chitosan-heparin nanoparticle coating on anodized NiTi for improvement of blood compatibility and biocompatibility[J]. Int J Biol Macromol, 2019,127:159-168. DOI: 10.1016/j.ijbiomac.2019.01.026.
[12] Lee SY, Lee CH, Kim DY, et al.Preparation and photocatalytic activity of potassium- incorporated titanium oxide nanostructures produced by the wet corrosion process using various titanium alloys[J]. Nanomaterials (Basel), 2015,5(3):1397-1417. DOI: 10.3390/nano5031397.
[13] 苏向东, 王天民, 郝维昌, 等. 低温去合金化处理对医用镍钛合金表面性质的影响[J].稀有金属材料与工程, 2008,37(5):859-863. DOI: 10.3321/j.issn:1002-185X.2008.05.026.
[14] Pound BG.The electrochemical behavior of nitinol in simulated gastric fluid[J]. J Biomed Mater Res B Appl Biomater, 2017,105(8):2394-2400. DOI: 10.1002/jbm.b.33779.
[15] Pound BG.Corrosion behavior of nitinol in blood serum and PBS containing amino acids[J]. J Biomed Mater Res B Appl Biomater, 2010,94(2):287-295. DOI: 10.1002/jbm.b.31647.
[16] Song T, Gao Y, Zhang Z, et al.Dealloying behavior of rapidly solidified Al-Ag alloys to prepare nanoporous Ag in inorganic and organic acidic media[J]. CrystEng Comm, 2011,13:7058-7067. DOI:10.1039/c1ce05538k.
[17] 崔跃, 张宝祥, 马连彩, 等. 镍钛合金在医疗器械领域应用和表面改性研究进展[J].材料导报,2017, 31(z2):197-200.
[18] Bacakova L, Filova E, Parizek M, et al.Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants[J]. Biotechnol Adv, 2011,29(6):739-767. DOI: 10.1016/j.biotechadv.2011.06.004.
[19] Hallab NJ, Bundy KJ, O'Connor K, et al. Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion[J]. Tissue Eng, 2001,7(1):55-71. DOI: 10.1089/107632700300003297.
[20] Rupp F, Liang L, Geis-Gerstorfer J, et al.Surface characteristics of dental implants: a review[J]. Dent Mater, 2018,34(1):40-57. DOI: 10.1016/j.dental.2017.09.007.
[21] Alipour S, Taromian F, Ghomi ER, et al.Nitinol: from historical milestones to functional properties and biomedical applications[J]. Proc Inst Mech Eng H, 2022,236(11):1595-1612. DOI: 10.1177/09544119221123176.

3D打印镍钛植入物表面去合金化处理及细胞相容性研究

Study on surface dealloying treatment and cytocompatibility of 3D printed nickel-titanium implants

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