The influence of vancomycin on osteogenic efficacy of BMP2-coprecipated biomimetic calcium phosphate granules

doi:10.12337/zgkqzzxzz.2019.06.001

Abstract

Abstract: Objective： To investigate the influence of vancomycin on osteogenic efficacy of BMP2-coprecipated biomimetic calcium phosphate granules. Methods： After being seeded on BioCaP-BMP2 granules, BM- SCs were treated with vancomycin at concentration of 0,1, 10, 30μg/ml. Cell proliferation was detected at 1, 4, 7 days by CCk8 assay. Total protein, alkaline phosphatase activity and osteocalcin expression were examined at 4 and 7 days. Results： Compared with control group, vancomycin at concentration of 1 and 10μg/ml did not show negative effects on the biological behavior of BMSCs. On the contrary, high concen- tration of vancomycin was found to inhibit the proliferation and osteogenic differentiation of BMSCs. Conclusion: Therapeutic concentration of vancomycin will not negatively influence the osteogenic efficacy of BioCaP-BMP2, which means BioCaP-BMP2 granules carrying vancomycin could be a promising material to treat infectious bone defects.

Key words: bone marrow mesenchymal stem cells, vancomycin, bone morphogenetic protein 2, os- teogenic differentiation

CLC Number:

783.1

WANG Dongyun, LUO Yuan, PENG Juanhong. The influence of vancomycin on osteogenic efficacy of BMP2-coprecipated biomimetic calcium phosphate granules[J]. Chinese Journal of Oral Implantology, 2019, 24(2): 51-54.

References

[1] Prathapachandran J and Suresh N. Management of peri-implantitis[J]. Dent Res J, 2012, 9(5) : 516-21
[2] Inzana J A, Schwarz E M, Kates S L, et al.Biomateri- als approaches to treating implant-associated osteomyelitis[J]. Biomaterials, 2015, 81 : 58-71
[3] Zheng Y, Wu G, Liu T, et al.A novel BMP2-copre- cipitated, layer-by-layer assembled biomimetic calcium phos- phate particle: a biodegradable and highly efficient osteoinducer[J]. Clin Implant Dent Relat Res, 2014, 16(5) : 643-54
[4] Liu T, Zheng Y, Wu G, et al.BMP2-coprecipitated calcium phosphate granules enhance osteoinductivity of deprot- einized bovine bone, and bone formation during critical-sized bone defect healing[J]. Sci Rep, 2017, 7 : 41800
[5] Wang D, Tabassum A, Wu G, et al.Bone regenera- tion in critical-sized bone defect enhanced by introducing osteo- inductivity to biphasic calcium phosphate granules[J]. Clin Oral Implants Res, 2017, 28(3) : 251-260
[6] Anagnostakos K.Therapeutic Use of Antibiotic-load- ed Bone Cement in the Treatment of Hip and Knee Joint Infec- tions[J]. J Bone Jt Infect, 2017, 2(1) : 29-37
[7] Tang J, Hu J, Kang L, et al.The use of vancomycin in the treatment of adult patients with methicillin-resistant Staphylococcus aureus (MRSA) infection: a survey in a tertiary hospital in China[J]. Int J Clin Exp Med, 2015, 8(10) : 19436-41
[8] Fleischman A N,Austin M S.Local Intra-wound Administration of Powdered Antibiotics in Orthopaedic Surgery[J]. J Bone Jt Infect, 2017, 2(1) : 23-28
[9] Kshetry A O, Pant N D, Bhandari R, et al.Minimum inhibitory concentration of vancomycin to methicillin resistant Staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in Nepal[J]. Antimicrob Resist Infect Control, 2016, 5: 27
[10] Geerlof L M and Boucher J. Evaluation of vancomy- cin dosing and corresponding drug concentrations in pediatric pa- tients. Hosp Pediatr[J]. 2014, 4(6) : 342-7
[11] Naschitz J E, Gagarin A,Schor R G, Spurious toxic vancomycin levels[J]. Eur J Intern Med, 2008, 19(6) : 36-7
[12] Buchholz H W and Engelbrecht H. [Depot effects of various antibiotics mixed with Palacos resins]. Chirurg, 1970, 41(11) : 511-5
[13] Bistolfi A, Massazza G, Verne E, et al.Antibioticloaded cement in orthopedic surgery: a review[J]. ISRN Orthop, 2011. 2011 : 290851