The study on properties of guided bone regeneration of new type chitosan-based thermosensitive hydrogel membranes in vivo

doi:10.12337/zgkqzzxzz.2019.03.001

Abstract

Abstract: Objective： To observe chitosan/β-glycerophosphate salt(CS/β-GP) thermosensitive hydrogel mem-branes loaded enamel matrix proteins and detect the guided bone regeneration properties. Methods： CS/β-GP membranes were synthesized in vitro and loaded with EMPs. 10 male and healthy Wistar rats wererandomly divided into two groups(A and B). Critical size defect(CSD) in a diameter of 5mm was created onboth sides of the skull of the rats. The experimental side on the left was covered by CS/β-GP(1.0g) com-posite membrane loaded EMPs on the left and the control side was covered by CS/β-GP (1.0g) membraneon the right. The bone reparation of the groups on both sides was compared by observation, density mea-surement of newly formed bone and hematoxylin-eosin staining. Results： The percentage of bilateralbone density measured at 1 month and 2 months had statistical significance(P< 0.05). HE staining showedthat there were a few new bone formation on both sides at 1 month after operation. At 2 months after opera-tion, the new bone formation in the experimental side increased and became more compact and mature.Conclusion： This kind of CS/β-GP composite membrane has the characteristics of guided bone regener-ation, and can significantly accelerate bone healing after loading bioactive factors.

Key words: chitosan, β-glycerophosphate salt, enamel matrix proteins, thermosensitive hydrogel, guided bone regeneration

CLC Number:

R783.6

LIANG Jie, ZHOU Ti, CUI Jun, et al. The study on properties of guided bone regeneration of new type chitosan-based thermosensitive hydrogel membranes in vivo[J]. Chinese Journal of Oral Implantology, 2019, 24(1): 1-4.

References

[1] Yan J, Yang L, Wang G, et al.Biocompatibility eval- uation of chitosan-based injectable hydrogels for the culturing mice mesenchymal stem cells in vitro[J]. Biomater Appl, 2010, 24(7) : 625-37
[2] 吴广升,张艺文. 壳聚糖温敏凝胶负载釉基质蛋白对骨髓基质细胞的作用[J]. 上海口腔医学, 2009, 18(2) : 178-182
[3] Shi W, Ji Y, Zhang X, et al.Characterization of ph and thermosensitive hydrogel as a vehicle for controlled protein delivery[J]. Pharm Sci, 2011, 100(3) : 886-895
[4] 王一波,李云鹏,侯锐,等. 人间充质干细胞在壳聚糖温敏凝胶中增殖和成骨分化的实验研究[J]. 口腔医学研究, 2015, 31(1) : 38-40
[5] CUI Jun, JIANG Bao-Qi,LIANG Jie, et al.Prepara- tion and Characterization of Chitosan/β-GP Membrane for Guid- ed Bone Regeneration[J]. Journal of wuhan university of technolo- gy-materials science edition, 2011, 2(26) :242-246
[6] In vitro and in vivo evaluation of chitosan/β-glycerol phosphate composite membrane for guided bone regeneration[J]. Jun Cui, Jie Liang, Yong Wen, Xiaoning Sun, Tiejun Li, Gairong Zhang, Kangning Sun, Xin Xu. J Biomed Mater Res A, 2014, 102(9) : 2911-2917
[7] 梁杰, 崔军,许胜, 等. 新型壳聚糖基引导骨再生膜的蛋白缓释性能研究[J]. 中国口腔种植学杂志, 2014, 19(04): 164-168
[8] 梁杰, 崔军, 许胜, 等.新型壳聚糖基温敏凝胶膜引导骨再生性能的体外实验研究[J]. 上海口腔医学, 2015, 24(04): 442-446
[9] 宋爱梅, 束蓉, 谢玉峰, 等.釉基质蛋白对猪骨髓基质细胞增殖和根面附着生长的影响[J]. 实用口腔医学杂志, 2006, 22(5): 679-683
[10] Schmitz JP, Hollinger JO.The critical size defect as an experimental model for craniomandibulofacial nonunions.[J]. Clin Orthop,1986, 205 : 299-308
[11] Hollinger JO, Kleinschmidt JC.The critical size de- fect as all experimental model to test bone repair materials.[J]. Craniofac Surg, 1990, 1(1):60
[12] Mhawi AA, Peel SA, Fok TC, et al.Bone regenera- tion in athymic calvarial defects with Accell Dbm100.[J]. J Cra- niofac Surg, 2007, 18(3) : 497-503
[13] Risbud MV, Bhonde MR, Bhonde RR.Effect of chi- tosan-polyvunyl prrolidone hydogel on proliferation and cyto- kine expression of endothelial cell :implications in islet immuno- isolation[J]. Biomed Mater Res, 2001, 57(2) : 300-305
[14] Miron RJ, Hedbom E, Ruggiero S, et al.Premature osteoblast clustering by enamel matrix proteins induces osteo- blast differentiation through up-regulation of connexin 43 and N-cadherin[J]. PLoS ONE, 2011, 6(8): e23375
[15] Miron RJ, Oates CJ, Molenberg A, et al.The effect of enamel matrix proteins on the spreading, proliferation and differ- entiation of osteoblasts cultured on titanium surfaces[J]. Biomate- rials, 2010, 31(3): 449-460
[16] Yoneda S, Itoh D, Kuroda S, et al.The effects of enamel matrix derivative(EMD)on osteoblastic cells inculture and bone regeneration in a rat skull defect[J]. J Periodont Res, 2003, 38 ; 333-342
[17] Ruel E, Chenite A,Chaput C,et al.Characterization of thermosensitive chitosan gels for the sustained delivery of drugs[J]. Int J Pharm, 2000, 203(8): 89-98