氧化石墨烯/氧化锌/纳米羟基磷灰石复合微球修复兔股骨髁骨缺损的实验研究

doi:10.12337/zgkqzzxzz.2025.08.002

中国口腔种植学杂志 ›› 2025, Vol. 30 ›› Issue (4): 331-340.DOI: 10.12337/zgkqzzxzz.2025.08.002

氧化石墨烯/氧化锌/纳米羟基磷灰石复合微球修复兔股骨髁骨缺损的实验研究

张国梁¹, 汪建国¹, 张宏宇¹, 张双圣¹, 吴江²

¹佳木斯大学附属口腔医院口腔颌面外科 154002;
²佳木斯大学附属口腔医院口腔解剖生理学教研室 154002

收稿日期:2025-01-22 出版日期:2025-08-30 发布日期:2025-08-29
通讯作者: 吴江,Email：22480384@qq.com,电话：0454-8625581
作者简介:张国梁,主任医师、副教授、口腔医学硕士,研究方向：口腔癌及医用生物材料。
吴江,副主任医师、副教授、医学博士,研究方向：医用生物材料。
基金资助:
黑龙江省自然科学基金项目（LH2023H002）;黑龙江省省属本科高校基本科研业务费科研项目（2022-KYYWF-0647）

Experimental study of GO/ZnO/nHAp composite microspheres in repairing femoral condylar bone defects in rabbits

Zhang Guoliang¹, Wang Jianguo¹, Zhang Hongyu¹, Zhang Shuangsheng¹, Wu Jiang²

¹Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang, China;
²Department of Oral Anatomy and Physiology, Affiliated Stomatological Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang, China

Received:2025-01-22 Online:2025-08-30 Published:2025-08-29
Contact: Wu Jiang, Email: 22480384@qq.com, Tel: 0086-454-8625581
Supported by:
Heilongjiang Natural Science Foundation Project (LH2023H002); Heilongjiang Provincial Undergraduate Colleges and Universities Undergraduate Research Business Expenses Research Project (2022-KYYWF-0647)

摘要/Abstract

摘要： 目的通过建立兔股骨髁标准临界骨缺损模型,复刻氧化石墨烯/氧化锌/纳米羟基磷灰石（GO/ZnO/nHAp）复合微球的体内骨再生情况,以评估其作为骨再生材料的可行性。方法采用离子凝胶-滴注法制备复合微球,将制备完成的氧化石墨烯/氧化锌（GO/ZnO）微球、纳米羟基磷灰石（nHAp）微球及GO/ZnO/nHAp复合微球材料分别植入3组实验兔股骨髁标准临界骨缺损模型中,并设空白对照组。术后4周、8周、12周分批处死动物,完整取材后进行大体观察、放射线检查、锥形束计算机体层成像（CBCT）检查、实时定量聚合酶链反应（qPCR）、蛋白印迹（Western blot）实验、硬组织切片组化染色及肝肾组织苏木精-伊红（HE）染色观察等评估,探讨植入材料的成骨性及骨结合情况。结果术后4周、8周、12周,同一时间点GO/ZnO/nHAp复合微球组经放射线检查、CBCT检查、qPCR及Western blot等检测,结果均明显优于GO/ZnO及nHAp微球组,且qPCR、Western blot及硬组织切片组化染色,定量研究结果组间比较采用单因素方差分析显示,3种微球材料在促成骨方面存在显著差异（P<0.05）。结论 GO/ZnO、nHAp、GO/ZnO/nHAp 3种微球材料均具有良好的生物相容性及一定的成骨活性。GO/ZnO/nHAp复合微球能有效修复兔股骨髁骨缺损,其体内骨传导、骨诱导性能明显优于GO/ZnO微球及nHAp微球,在骨缺损修复材料方面有潜在应用价值。

关键词: 骨再生材料, 复合微球, 成骨诱导, 临界骨缺损

Abstract: Objective To evaluate the possibility of GO/ZnO/nHAp composite microspheres as bone regeneration materials, a critical bone defect model of the rabbit femoral condyle was established to access bone regeneration in vivo. Methods Composite microspheres were prepared by the ionic gel-drip method, and the prepared GO/ZnO microspheres, nHAp microspheres, and GO/ZnO/nHAp microspheres were implanted into three groups of experimental rabbit femoral condyle standard critical bone defect models, with a blank control group. The animals were killed in batches at 4 weeks, 8 weeks, and 12 weeks after the operation, and the samples were taken completely for evaluation. Gross observation, X-ray film, CBCT detection, qPCR, Western blot, hard histological section, and HE staining of liver and kidney pathology were carried out to explore the osteogenesis and osseointegration of implanted materials. Results At 4 weeks, 8 weeks and 12 weeks after operation, the results of X-ray, CBCT, qPCR and Western blot in the GO/ZnO/nHAp composite microsphere group were significantly better than those in GO/ZnO and nHAp microspheres group at the same time point. The comparison of the experimental results of the qPCR, Western blot and hard tissue section showed that there were statistically significant differences among the three microspheres in promoting bone formation (P<0.05). Conclusion GO/ZnO, nHAp, and GO/ZnO/nHAp all have good biocompatibility and osteogenic activity. GO/ZnO/nHAp composite microspheres can effectively repair the bone defect of rabbit femoral condyle, and their in vivo bone conduction and bone induction properties are superior to GO/ZnO microspheres and nHAp microspheres, which have potential application value in bone defect repair materials.

Key words: Bone regeneration materials, Composite microspheres, Osteoinduction, Critical size defect

张国梁,汪建国,张宏宇,等. 氧化石墨烯/氧化锌/纳米羟基磷灰石复合微球修复兔股骨髁骨缺损的实验研究[J]. 中国口腔种植学杂志, 2025, 30(4): 331-340. DOI: 10.12337/zgkqzzxzz.2025.08.002

Zhang Guoliang, Wang Jianguo, Zhang Hongyu, Zhang Shuangsheng, Wu Jiang. Experimental study of GO/ZnO/nHAp composite microspheres in repairing femoral condylar bone defects in rabbits[J].Chinese Journal of Oral Implantology, 2025, 30(4): 331-340.DOI: 10.12337/zgkqzzxzz.2025.08.002.

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氧化石墨烯/氧化锌/纳米羟基磷灰石复合微球修复兔股骨髁骨缺损的实验研究

Experimental study of GO/ZnO/nHAp composite microspheres in repairing femoral condylar bone defects in rabbits

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